Uv-Visible Spectroscopy
UV-Visible/NIR spectroscopy (UV-Vis Spectroscopy) can be divided into ultraviolet, visible, and near-infrared regions of the spectrum, depending on the wavelengths used. since its frequency is close to the overtone frequency of many natural vibrations, weak substance-specific absorption bands can be detected. It can therefore be used for non-destru
Principle of Uv-Vis Spectroscopy
UV-Visible spectroscopy exploits the wave-like nature of electrons and its interactions with electromagnetic radiation. UV-Vis spectroscopic instruments are used to identify, characterize, and quantify an extraordinary wide range of molecular compounds. It can be used for non-destructive measurements, such as determining the sugar, lipid, protein c
Beer-Lambert Law
A UV-Visible/NIR spectrophotometer measures the transmittance or the amount of light transmitted through a sample by ratioing the intensity of the incident light (I0) to the intensity of the transmitted light (I). T= I/I0 The relationship between transmittance and absorbance is described by the following equation abs = 2-log I/I0∙100 = 2-log (%T)