Hyperspectral imaging (HSI) and multispectral imaging (MSI) technologies have the potential to transform the fields of digital and computational pathology. Traditional digitized histopathological slides are imaged with RGB imaging.
Abstract: Hyperspectral images (HSIs) have very high spectral resolution, which can reflect the characteristics of different materials well. However, compared with RGB image or multispectral image (MSI), the spatial resolution of HSI is much lower, which limits its applications.
For the sake of comprehension, the background of Hyperspectral Images and segmentation techniques is briefly discussed at first. The study is organised around different Hyperspectral Image processing techniques such as thresholding, clustering, watershed, deep learning, and other methods.
The Nuance CRi Multispectral Imaging System (Cambridge Research and Instrumentation, Woburn, MA; PerkinElmer, Inc., Hopkinton, MA) is available in three versions (VX, FX, and EX), which capture MS images in the wavelength ranges of 420 to 720 nm (VX and FX) and 450 to 950 nm (EX).