[PDF] Cell Biology - Eppendorf





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[PDF] Cell Biology - Eppendorf

Cell Biology is a branch of biology that studies cells — the organelles they contain subfields; the study of cell metabolism, the study of cellular genetics and the

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[PDF] Cell Biology - Eppendorf 32185_7Workflow_Problems_Solution_Folder_CellBiology_RZ_1_.pdf

Improve your laboratory workflow

Cell Biology

Cultivation Under

Controlled Conditions

Cell Biology is a branch of biology that studies cells - the organelles they contain, their function, their physiological properties, their life cycle and the interactions with their environment. Cell Biology basic research can be divided into several subfields; the study of cell metabolism, the study of cellular genetic s and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell commun ication and signaling. Research in cell biology overlaps to a great extent other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology. Our Cell Culture Consumables are developed to facilitate the lab work while offering safety and convenience in handling. With our comprehensive range of bioprocess solutions cells can be cultivated under controlled conditions, e.g. using single-use vessels.

Cell cultivation

How do I prevent

inhomogeneous cell growth?

What about protection against

contamination?

Inhomogeneous cell adhesion!

What now?

How to avoid negative e?ects

caused by leachables or extractables?

Cell culture plates; CO

2 incubatorsCell culture dishes; CO 2 incubatorsMost frequent problems /challenges

Most frequent problems /challenges

Most frequent problems /challenges

Most frequent problems /challengesPossible solution

Possible solution

Possible solution

Possible solutionEppendorf's solutionEppendorf's solutionEppendorf's solutionEppendorf's solution

Pipettes and tips; Dishes, flasks and plates

Bioprocess systems; Single-use vessels

Eppendorf's product benefits:

> Peripheral moat in 96-well Cell Culture Plates to minimize edge e?ect > Possibility to fill the inter well space of all Cell Culture Plate formats to reduce temperature variations > Usage of split inner doors of Galaxy® CO 2 incubator for stable growth conditionsEppendorf's product benefits: > Easy to clean incubators with high temperature disinfection mode > Corrugated handling ring of Cell Culture Dishes for safe transportation > SplashProtect™ ring in dishes to avoid splashes and bridging of medium with outside environment > Flasks with optimized geometry Minimize the risk by: > Perform incubator disinfection routine regularly >Fill consumables one by one > Use flasks with a screw cap instead of dishes with a loose lid e. g. for maintenance of stock cultures or when frequent transportation of the cultures is needed

Minimize the risk by:

>Omit usage of the edge wells > Increase sedimentation time for cells in the 96-well plate at room temperature > Use a separate CO 2 incubator for cells seeded for assays > Minimize door opening events of the CO 2 incubator

Minimize the risk by:

> Regular resuspension of cells during seeding > Manual movement of consumable after seeding > Cell culture consumables with high quality standard

Minimize the risk by:

> Extractables studies to identify and quantify potentially harmful compounds >Rigid-wall stirred tank bioreactorsContamination of cells > The CO 2 incubator can be a source of contamination > Medium and cells in contact with outside air due to splashing and inadvertent removal of the lid

Inhomogeneous cell growth

> Inhomogeneous distribution of cells in the edge wells of multiwell plates >Higher evaporation rate in edge wells >Temperature variations during incubation > Variations in chamber atmosphere due to door opening of the CO 2 incubator

Inhomogeneous cell adhesion

>Variations in seeding cell numbers > Formation of air bubbles during the seeding process >Uneven distribution of cells

Issues related to leachables & extractables

>Cytotoxic e?ects >Altered product characteristicsEppendorf's product benefits: > Fast seeding operation using

Multipette®

(U.S. / CAN: Repeater ® ) and Combitips ® >Dispension steps for avoiding air bubble insertion > Batch tested and certified consumables for consistent quality

Eppendorf's product benefits:

> Single-use vessels produced from monolayer injection-molded plastics: materials comply with

USP Class VI

> Virgin raw materials, all sourced by Eppendorf directlyCultivation Under

Controlled Conditions

Cell Biology is a branch of biology that studies cells - the organelles they contain, their function, their physiological properties, their life cycle and the interactions with their environment. Cell Biology basic research can be divided into several subfields; the study of cell metabolism, the study of cellular genetic s and the underlying regulatory mechanisms, the study of cell compartment structures, the study of cell cycle, division and death and the study of cell commun ication and signaling. Research in cell biology overlaps to a great extent other areas of biology and chemistry, particularly genetics, biochemistry and molecular biology. Our Cell Culture Consumables are developed to facilitate the lab work while offering safety and convenience in handling. With our comprehensive range of bioprocess solutions cells can be cultivated under controlled conditions, e.g. using single-use vessels.

Cell cultivation

www.eppendorf.com/workflows

Eppendorf

® , the Eppendorf logo, Biopur ® , Multipette ® , Repeater ® , Combitips ® , and Combitips advanced ® are registered trademarks of Eppendorf AG, Germany. New Brunswick™ is a trademark of Eppendorf AG, Germany. Galaxy ® is a registered trademark of Eppendorf Inc., USA.

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® is a registered trademark of DASGIP Information and Process Technology GmbH, Germany.

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eppendorf@eppendorf.com · www.eppendorf.com Eppendorf North America, Inc. · Phone: 800-645-3050 info@eppendorf.com · www.eppendorfna.com

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Being in Process

StorageAnalysis and detecptionHarvesting and preparationCell cultivationCell manipulation

Cell seeding

Cell Biology

(Eukaryotic cells) FreezersDeepwell platesConical tubesPCR consumablesThermocyclersPhotometersCell imaging consumapbles

Sealing optionsConcentratorCuvettesBioreactors

CentrifugesCO

2 incubators

Tubes and platesPipettes and tips

Thermo mixing devicespBioprocess systems

Biopur

® consumablesDishes, flasks and pplates

Conical tubesMicromanipulators

Pipette controllersMicroinjectorsCapillaries

AutomationDishes, flasks and pplatesSerological pipettesCentrifugesPhotometers

Conical tubes


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