DNA staining for cell cycle (adapted from Zbignew Darynkiewicz's method): 7 Re-suspend the cells in 5 ml PBS, wait 60 seconds and centrifuge the cells
DNA%20staining%20for%20cell%20cycle.pdf
Commonly used DNA dyes used for flow cytometric cell cycle analysis include Fixed Cell Staining Protocols for PI, 7-AAD, ToPro-3 and DAPI
Cell%20Cycle%20Tutorial.pdf
5 nov 2020 · Cell cycle profiling by flow cytometry using 7-AAD for DNA content analysis Jurkat cells were fixed with ice-cold methanol, then stained with
PI-40084-40037v11.5.2020.pdf
This method allows the analysis of cell cycle profile of live cells stained for specific 7AAD/Saponin solution: 0 03 Saponin, 25?g/ml 7AAD, 1 BSA
facilities-flowcyt-7aad_cell_cycle_of_non-fixed_cells.pdf
PI/7-AAD or Live/Dead Fixable stains Protocol: Prepare Cells: • Collect cells and transfer approximately 1 x 106 cells/mL into a 5mL (12 x 75 mm tube)
Sufrace_Staining-_-Live_Cell_Cycle.pdf
PI/7-AAD or Live/Dead Fixable stains Protocol: Prepare Cells: • Collect cells and transfer approximately 1 x 106 cells/mL into a 5mL (12 x 75 mm tube)
Sufrace-Staining-with-Cell-Cycle-fixed.pdf
Cell cycle analysis by quantitation of DNA content was one of the earliest applications 7-aminoactinomycin-D (7-AAD), Hoechst 33342 and 33258, TO-PRO-3,
pi.pdf
Hoechst concentration is needed for live cells Several companies have cell cycle dyes for live cells o DAPI, Hoechst 33258, 7-AAD • Cell impermeable
Celle_cyklus__FINAL_2.pdf
are combined with DNA dyes such as 7-AAD, it is possible to resolve cell cycle phases in a given cell cell cycle analysis using flow cytometry
BD_FACSVerse_CellCycleAnalysis_AppNote.pdf