The excitation of PI at 488 nm facilitates its use on the benchtop cytomters Protocol for staining whole cells with PI: 1 Harvest cells and prepare single
DNACellCycleAnalysiswithPI.pdf
There are numerous fluorescent DNA dyes used for multiple purposes not all are suitable for flow cytometric cell cycle analysis Only fluorescent DNA dyes
Cell%20Cycle%20Tutorial.pdf
analysis without further optimisation (see “Internal antigens and cell cycle” protocol Materials 1 70 ethanol (in DI water) 2 1x PBS
facilities-flowcyt-pi_cell_cycle.pdf
CellCycle Analysis The cell cycle profile of a sample can be determined by staining the DNA with a fluorescent dye and PI cell cycle staining protocol:
cell-cycle-analysis-pi.pdf
Cell cycle analysis – Propidium iodide (PI) 1 Harvest approx 1^106 cells and centrifuge at 400 g for 5 min 2 Wash cells once with cold PBS
cell_cycle_analysis_0.pdf
Cell Cycle Analysis by Propidium Iodide (PI) Staining Adherent cells: 1 trypsinized 2 suspended in medium + 10 FCS 3 centrifuged (1000 rpm, 5 min)
Cell_Cycle_with_PI.pdf
The cell cycle is a ubiquitous, complex sequence of events leading to growth and proliferation of cells The cell cycle progression is tightly regulated due to
mak344bul.pdf
For cells that must be stained prior to EtOH fixation, stain the cells according to the manufacturer's protocol 3 Wash cells in staining buffer before
Cell-Cycle%20Analysis%20Using%20the%20BD%20FACSArray%E2%84%A2%20Bioanalyzer.pdf