DAPI staining for cell cycle analysis Binds preferentially A-T base regions in DNA Process between 1 - 10 million of cells 1) Spin the cells at 500g,
dapi-cell-cycle-analysis.pdf
The protocol below may be used for DAPI measurements alone and should not be combined with other dyes or surface markers since alcohol based fixatives degrade
CellCycle_-DAPI_Alcohol.pdf
Protocol: Cell Cycle Staining-DAPI Cell Prep and Staining 1 Harvest cells - wash 2X in PBS to get rid of serum proteins 1200rpm, 5
DAPI-Protocol.pdf
Commonly used DNA dyes used for flow cytometric cell cycle analysis include propidium iodide (PI), 4',6-Diamidino-2-phenylindole dihydrocloride (DAPI),
Cell%20Cycle%20Tutorial.pdf
The sample must be fixed and permeabilized to allow the dye to enter the cells • Propidium Iodide (PI) – Blue/Green lasers, also stains RNA • DAPI – UV/
Flow_TechNotes_Cell-cycle-analysis_20170918.pdf
DAPI 345 455 Invitrogen Hoechst 33342 350 461 Invitrogen DyeCycle Violet* 369 437 Invitrogen Propidium Iodide (PI) 536 617 Invitrogen 7-AAD 546
DNA%20staining%20for%20cell%20cycle.pdf
550913) according to the kit protocol After washing, cells were stained with 1 µg/mL BD Pharmingen DAPI Solution for DNA content Cyclin A expression can be
Cell-Cycle-Proliferation-Kits-DS.pdf