[PDF] auto induction definition

What does auto induction mean?

auto-induction; Etymology . From auto-+? induction. Noun . autoinduction (uncountable) (biochemistry) The expression of an enzyme that is responsible for the metabolism of the agent expressing it; Related terms . autoinducible

Is autoinduction a labor-saving method?

With advent of the Protein Structure Initiative, autoinduction was introduced as a labor-saving method, and an extensive description of the development of autoinduction for use with the pET system was reported ( Studier, 2005) and adapted to the use of T5 promoter plasmids ( Sreenath et al., 2005; Tyler et al., 2005 ).

What is autoinduction medium?

The autoinduction medium described in this unit contains glucose, lactose, and other carbon sources. When lactose becomes the preferred carbon source, it is imported into the cell and can simultaneously support growth of the host cells and induce expression of recombinant proteins.

When was autoinduction first discovered?

The term "autoinduction" was first coined in 1970, when it was observed that the bioluminescent marine bacterium Vibrio fischeri produced a luminescent enzyme ( luciferase) only when cultures had reached a threshold population density. At low cell concentrations, V. fischeri did not express the luciferase gene.

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