[PDF] 30 sucrose cryoprotectant recipe

Final cryoprotectant concentration will be: 30% ethylene glycol, 30% sucrose, 0.00002% sodium azide, in 0.1 M PB (pH 7.35).
View PDF Document


  • How do you make a 30% sucrose solution?

    Prepare 30% sucrose solution: 15 g sucrose + 35 mL 10 mM PBS in a 50-mL Falcon tube. Place the tube on a rocker to dissolve sucrose and then keep at 4°C. Alternatively, 30% sucrose can be prepared in a larger quantity, filtered through a 0.22 ?m and stored at 4°C for 1 month.

  • How long can tissue stay in 30% sucrose?

    Alternatives: Tissue can be left immersed in 30% sucrose at 4°C for up to 5 days.

  • How does sucrose work as a Cryoprotectant?

    Nonpermeable cryoprotectants, such as sugars (e.g., trehalose and sucrose), starches (e.g., hydroxyethyl starch), polyvinyl pyrrolidone, and polyethylene oxide, cannot enter cells and thus stay extracellular during cryopreservation. They are normally applied to protect cells during rapid cooling.
  • Cryopreservation of LDL in 10% sucrose (w/v) completely prevented the structural and functional changes incurred after short-term freezing, and LDL cryopreserved in sucrose for as long as 18 months displayed cell binding, uptake, and degradation very similar to that of freshly obtained LDL.
View PDF Document




Tissue preparation and cryopreservation with sucrose -- for frozen

16 déc. 2016 Cryoprotectants are particularly important when freezing large tissue samples such as rodent brains. Common cryoprotectants used to preserve ...



Cryostat Procedure

1 nov. 2012 Attach a wire into holes to create a handle. • After sample has incubated in 30% sucrose overnight acquire liquid nitrogen. Fill container.



A cryoprotection method that facilitates cutting frozen sections of

Cutting frozen sections of large (>60 cc) blocks of monkey brain using the conventional procedures ofinfiltration with. 30% sucrose as a cryoprotectant.



Synaptic Systems standard protocol for tissue preparation for IHC

Cryoprotectant solution: 30% sucrose 1% Polyvinyl-pyrrolidone (PVP-40)







The effect of paraformaldehyde fixation and sucrose cryoprotection

22 nov. 2013 metal levels throughout a complete fixation/cryoprotection protocol. Blank-corrected metal levels in 4%. PFA and two changes of 30% sucrose ...



Transcardial Perfusion of Mice for Immunohistochemistry

14 oct. 2016 Cryoprotection. 17) Place fixed brain in a 50 mL conical tube containing ice-cold (4oC) 10% sucrose solution (recipe.



A Short Review on Cryoprotectants for 3D Protein Structure Analysis

19 jan. 2022 Sucrose. Maltose. Xylitol. Inositol. Trehalose. Raffinose. Erythriol ... Indeed glycerol



CHMP assessment report on group of an extension of marketing

14 oct. 2021 Tris/Sucrose 30. µg roll 2 (Line. Extension). September. 2021. Batch analysis