[PDF] acid hydrolysis fat determination

Crude fat is determined by acid hydrolysis with HCl (25 + 11) followed by extraction of hydrolyzed lipid materials with mixed ethers. The solvent mixture is evaporated, and the residue is re-extracted and filtered with four, 10-ml portions of ethyl ether.
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  • What is the acid digestion method for fat analysis?

    For foodstuffs, the most commonly used method for determining the total fat content is extraction using acid digestion, i.e. the Weibull-Stoldt/Weibull-Berntrop method. The sample is heated up with hydrochloric acid in order to digest proteins and release the bound lipids.

  • What is the AOAC method for fat?

    The “Soxhlet” method described here is recognised by the Association of Official Analytical Chemists (AOAC) as the standard method for crude fat analysis. In addition, some rapid instrumental methods are also approved by the AOAC.

  • What are the methods for analysis of fat?

    These methods are based upon the ease of removing fat from a given matrix, and the selected method also depends on whether fat or total lipids are to be extracted from the sample. There are two common methods used to test and analyze fat in food: Gas chromatograph (GC) method. Solvent extraction-gravimetric method.
  • Acid hydrolysis is a hydrolysis mechanism in organic chemistry in which protic acid is used to catalyse the cleavage of a chemical bond by means of a nucleophilic replacement reaction with the addition of water elements (H2O).
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Crude Fat Methods – Considerations

Lipids may be fatty acids (bound or free) and derivatives phospholipids



Extraction of Total Fat From Food Samples After Acid Hydrolysis

hydrolysis or pretreatment prior to solvent extraction. Pretreatment or hydrolysis of these determination of fat content by FAME (Fatty Acid Methyl.



Extraction of Total Fat From Food Samples After Acid Hydrolysis

hydrolysis or pretreatment prior to solvent extraction. Pretreatment or hydrolysis of these determination of fat content by FAME (Fatty Acid Methyl.



Analysis of Total Fat

Soxhlet extraction Vs. Acid hydrolysis. GB/T 5009.6-2003. Determination of fat in foods. ? Soxhlet extraction. Crude fat including free fat (????).



Fat by Acid Hydrolysis

This procedure is applicable for the determination of crude fat in dried forages and mixed feeds. It is not applicable for oilseeds baked and/or expanded 



Determining Total Fat Content by Automated Acid Hydrolysis

or alkaline hydrolysis of fatty acids bound to glycerides SOXTHERM (rapid soxhlet extraction)



Total fat content by automated acid hydrolysis (HYDROTHERM

losed acid hydrolysis system by the ISO 8262-1. Weibull-Berntrop gravametric method. Paired with the SOXTHERM® (rapid soxhlet extraction) the fat analysis 



SN 007-2009 E-916 hydro food and feed_p

Fat Determination in Food and Feed Products using Speed-. Extractor E-916 after Acid Hydrolysis. The determination of fat in food and feed is a routine 



Composition of Fatty Acid and Identification of Lauric Acid Position in

The distribution of lauric acid in TAG was conducted through hydrolysis by using specific lipase enzymes active at sn-13 positions



Evaluation of Extraction Methods for Recovery of Fatty Acids from

2 Feb 2010 The extraction efficiency of Soxhlet acid hydrolysis and Bligh and ... The precision of fatty acid analysis by direct methylation method ...