[PDF] Users Guide GraphPad Prism User's Guide.

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User's Guide

Version 3

The fast, organized

wa y to analyze and graph scientific data ãã 1994-1999, GraphPad Software, Inc. All rights reserved. GraphPad Prism, Prism and InStat are registered trademarks of GraphPad Software, Inc. GraphPad is a trademark of GraphPad Software, Inc. Use of the software is subject to the restrictions contained in the accompanying software license agreement.

How to reach GraphPad Software, Inc:

Phone:858-457-3909 (619-457-3909 before June 12, 1999) Fax:858-457-8141 (619-457-8141 before June 12, 1999) Email:support@graphpad.com or sales@graphpad.comWeb:www.graphpad.comMail:GraphPad Software, Inc.

5755 Oberlin Drive #110

San Diego, CA 92121 USAWhen you opened the disk envelope, or downloaded the purchased software, you agreed to the Software License Agreement reprinted on page 197. GraphPad Software, Inc. does not guarantee that the program is error-free, and cannot be held liable for any damages or inconvenience caused by er- rors. Although we have tested Prism carefully, the possibility of software errors exists in any complex computer program. You should check important results carefully before drawing con- clusions. GraphPad Prism is designed for research purposes only, and should not be used for the diagnosis or treatment of patients. If you find that Prism does not fit your needs, you may return it for a full refund (less shipping fees) within 90 days. You do not need to contact us first. Simply ship the package to us with a note explaining why you are returning the program. Include your phone and fax numbers, and a copy of the invoice. If you purchased Prism through a dealer or catalog, please con- tact the company you purchased it from regarding returns.

Contents

Contents......................................5

Orientation....................................9

Introducing GraphPad Prism..............................9 System requirements...........................................9 Updating from a previous version.................................10 How Prism is organized............................................10 Starting GraphPad Prism..................................................11 Hints for people who don't read manuals..................................13 Tutorial................................................................15 Step 1. Start Prism.........................................................15 Step 2. Import data........................................................17 Step 3. Make a graph....................................................18 Step 4. Fit a curve.........................................................20 Step 5. Create a second graph................................................22 Step 6. Arrange two graphs on a page layout..............................24 Step 7. Embed a table....................................................26 Step 8. Exclude a value and update the graph............................27 Step 9. Copy and paste to PowerPoint.......................................28 Entering and editing data................................................29 Overview of data tables...................................................29 The Welcome dialog.....................................................30 Column formats............................................................31 Creating additional data tables.............................................34 Working with the data table...................................................35 Using the clipboard......................................................37 Entering multiple sets of data that don't share X values..........................38 Embedding data tables in graphs or layouts.................................39 Importing and pasting data..................................................41 Overview of importing and pasting...........................................41 Using data from Excel....................................................41 Using data from other spreadsheet or statistics programs........................45 The Format Data Object dialog.................................................46 Data objects in Prism......................................................52 Analyzing data...........................................................55 Data analysis overview.....................................................55 Choosing an analysis.......................................................56 Viewing and graphing results....................................................59 Changing an analysis......................................................60

Frozen and orphaned results...................................................61Analyzing repeated experiments............................................62

Saving templates and methods..................................................64 Analysis options...........................................................66 Graph types...............................................................67 Automatic graphs..........................................................67 XY graphs.....................................................................68 Column graphs...............................................................73 Bar graphs with two grouping variables.....................................74 Bar graphs with one grouping variable.......................................76 Changing the graph type..........................................................79 Creating graphs.........................................................81 Making a new graph.............................................................81 Graph options................................................................81 Create a series of similar graphs.............................................82 Choosing data to graph..................................................83 The Change button...............................................................86 Axes..........................................................................87 Overview of axes..........................................................87 Formatting axes............................................................87 Custom ticks.................................................................91 Logarithmic axes...........................................................94 Discontinuous axes.......................................................98 Hiding axes and showing scale bars..........................................101 Graphs with two Y-axes....................................................102 Thickness and color of axes...............................................104 Axis titles and numbering.......................................................104 Changing bars and symbols.................................................107 Format symbols and lines...............................................107 Format bars................................................................109 Format Columns dialog.....................................................113 Legends..........................................................................116 Error bars................................................................117 Overview............................................................................117 Displaying variability directly.................................................117 Statistical theory: SD, SEM and 95% CI....................................119 Error bars on XY graphs and bar graphs.....................................121 Error bars on column bar graphs.............................................123 Page layouts............................................................127 An overview of page layouts...................................................127 Changing the number or arrangement of graphs............................127 Assigning graphs to placeholders............................................129 Master legends...........................................................131 Arranging graphs...........................................................132 Colors.............................................................................135 Changing graph colors...................................................135 Background colors for graphs.....................................................136 Background colors for layouts.................................................136quotesdbs_dbs2.pdfusesText_2

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