[PDF] ChemStation E-Seminars 10 дек. 2008 г. VB. BB. BV.





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  • What is Methods and events in VB?

    Every Visual Basic control consists of three important elements ? Properties which describe the object, Methods cause an object to do something and. Events are what happens when an object does something.
  • What is the difference between event and method in VB?

    A method is nothing but a function which executes something in it when called. it can be called any time. A event is a result of a action performed by the user like click, hover, drag, re-size etc. There are event handlers.
  • What are Methods of Visual Basic?

    In Visual Basic, there are two ways to create a method: the Sub statement is used if the method does not return a value; the Function statement is used if a method returns a value. A class can have several implementations, or overloads, of the same method that differ in the number of parameters or parameter types.
  • Events are basically a user action like key press, clicks, mouse movements, etc., or some occurrence like system generated notifications. Applications need to respond to events when they occur. Clicking on a button, or entering some text in a text box, or clicking on a menu item, all are examples of events.

ChemStation E-Seminar

Dec, 2008

Page 1

ChemStation E-Seminars

ChemStation E-Seminar

Dec, 2008

Page 2

ChemStation E-Seminar Agenda Schedule

Short description

15. Oct. 2008

ChemStation Data Storage ConceptChemStation Navigation Table

How to improve Review and ReprocessIntegrating My Results in ChemStationHow to set up a calibration table in ChemStation

04. Feb. 2009

Various Reporting in Chemstation

Steven Brown

Ortrud Emde

Updated Compliance 21 CFR Part 11 solution with

Agilent ChemStation & OpenLAB ECM

12. Nov. 200810. Dec. 200814. Jan. 2009

Steven Brown

18. Feb. 2009

Agilent

SpeakerSteven Brown

Ortrud EmdeSteven BrownOrtrud EmdeSteven BrownOrtrud EmdeSteven BrownOrtrud Emde

ChemStation E-Seminar

Dec, 2008

Page 3

Seminar 3: Integrating my Results in ChemStation

TopicsThe Integration TaskGeneral Integration Items and the use of auto integrationInitial Integration EventsTimed EventsManual Integration eventsManual integration events and where to save them

ChemStation Rev. A / Rev. B.02.01 - B.03.02 / B.04.01

ChemStation E-Seminar

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The Integration Task

ChemStation E-Seminar

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Page 5

The Integration Process consists of the following: construct initial baseline peak recognition - start and end time find the apex of the peak baseline allocation (construction) peak area measurement

Integration Process

ChemStation E-Seminar

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1 Slope and Curvature within limit

2 Slope and curvature above limit: peak?

3 Slope remains above limit: peak recognized

4 Curvature becomes negative: front inflection

5 First Derivate zero: Apex

6 Curvature becomes positive: rear inflection point

7 Slope and curvature within limit: end of peak?

8 Slope and curvature within limit: end of peak

5 12 346
7

Start, End and Apex of a Peak

8

ChemStation E-Seminar

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To Integrate a Chromatogram, the Integrator:1) Defines the initial baseline.2) Continuously tracks and updates the baseline.3) Identifies the start time for a peak and marks this point with a vertical

tick mark.

4) Finds the apex of each peak, creates a parabolic fit for the peak top,

and stores the retention time.

5) Identifies the end time for the peak, and marks this point with a vertical

tick mark.

6) Constructs a baseline.7) Calculates the area, height, and peak width for each peak.

ChemStation E-Seminar

Dec, 2008

Page 8

BB BV VB BB BV VB

Tick Marks

Baseline

The default baseline is constructed as a series of straight line segments between: the start of the baseline, the tick marks, and the end of the peak.

Default Baseline Construction

ChemStation E-Seminar

Dec, 2008

Page 9

Integrator UI

Define integration

parameters just by selecting and clicking on the chromatogram

There are two sets of detector specific integration events:initial eventsand time-basedIntegration events for all signals

ChemStation E-Seminar

Dec, 2008

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When a Signal is Loaded, Integration May Occur

Automatically

Integrate by:

•Selecting Integrate after load in Load

Signaldialog box or

Preferencesdialog

box. •Selecting Integrateor

Auto Integrate from

the menu. •Selecting the

Integration or Auto

Integration Tool.

•Running a method where the Run

Time Checklist

includes Data Analysis.

ChemStation E-Seminar

Dec, 2008

Page 11

Auto Integrate

Examines beginning and end regions to estimate noise. Assigns initial Slope Sensitivity and Height Reject. Assigns temporary Peak Width value for first pass integration.

Sets Area Reject to zero.

Performs trial integration, may be repeated several times. Calculates Peak Width based on early eluting peaks.

Refines Slope Sensitivity and Height Reject.

Computes Area Reject as 90% area of most symmetrical peak.

Autointegrates based on your

settings in For All Signals.

Can be a good starting point

for integration events.

ChemStation E-Seminar

Dec, 2008

Page 12

Set Up Integration

Set up integration for your method in the Data

Analysis mode using a representative

chromatogram.

Loads IntegrationEvents

ChemStation E-Seminar

Dec, 2008

Page 13

Initial Integration Events

ChemStation E-Seminar

Dec, 2008

Page 14

Initial integration events

Use:Slope Sensitivityto define peak sensitivity

Peakwidthto set an initial sampling interval for the integrator to distinguish peaks from baseline noise

Area Rejectto filter small peaksHeight Rejectto set noise rejectionShoulderto specify the algorithm for shoulder detection

ChemStation E-Seminar

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Page 15

Initial Settings - Events

Peak Width- controls the ability of

the integrator to distinguish peaks from baseline noise. In general, increasing the peak width will result in broader peaks.

Area reject- All peaks whose

areas are below this value will not be reported.

Height reject- All peaks whose

heights are below this value will not be reported.

0.05 0.1

Slope sensitivity- decreasing slope

sensitivity will result in detecting smaller and broader peaks.

Shoulder Detection Mode- shoulders

detected using the second derivative of peak

ChemStation E-Seminar

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Shoulders occur when two peaks are so close together that no valley exists between them

Peak with shoulders

Tangent Shoulders

Drop Line Shoulders

Shoulder Detection

ChemStation E-Seminar

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Page 17

Initial Settings - For All Signals

Tangent Skim Mode- only applies whenconditions for the following settings are met: Tail Peak Skim Height Ratio, orFront Peak Skim Height Ratio, andSkim Valley Ratio

Peak To Valley Ratio

Baseline Correction must beAdvanced

Some Events in this table are dependent

on other events in this table.

ChemStation E-Seminar

Dec, 2008

Page 18

Tangent Skim Mode

•Tangent Skim Modes •New Exponential •Exponential •Straight •Standard The Tail Peak Skim Height RatioandSkim Valley Ratiowill be used to determine whether a tangent skim will be applied to calculate the area of a child peak on the trailing edge of a parent peak.

ChemStation E-Seminar

Dec, 2008

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Tail Peak Skim Height Ratio

H p H c H p H c

Ratio must be

greater than set value to be skimmed

Setting the value to zerodisablestangent skimming

ChemStation E-Seminar

Dec, 2008

Page 20

Tail Peak Skim Height Ratio

No Tangent Skimming

Tangent Skimmed

Tail Peak Skim Height Ratio = 3

Skim Valley Ratio = 20

Tail Peak Skim Height Ratio = 0

Skim Valley Ratio = 20

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