Isolation of Full Size BAC Inserts by DNA Gap Repair - bioRxiv
Cité 1 fois — When a gap is present in a cloning vector, DGR will allow a new DNA sequence to be
Recombineering & Gap Repair Protocol
retrieved using gap repair and turned into a gene targeting vector The BAC The protocol proposed uses electroporation to transfer the BAC DNA into SW102 Alternatively
P[acman]: A BAC Transgenic Platform for Targeted Insertion of
Cité 726 fois — Gap repair efficiently retrieves DNA fragments up to 133 kilobases long from P1 or BAC clones fC31-
P[acman]: A BAC Transgenic Platform for Targeted - Science
2006 · Cité 726 fois — Gap repair efficiently retrieves DNA fragments up to 133 kb from P1 or BAC clones
Review Article Bacterial Artificial Chromosome Mutagenesis
Cité 36 fois — (d) Gap repair cloning Figure 1: General applications of recombineering in BAC modifications
Copeland-2001 Nat rev Genetpdf - RECOMBINEERING
2001 · Cité 908 fois — BAC DNA Regions of homology Target gene Figure 4 Retrieving cloned DNA by gap repair
Lambda Red Mediated Gap Repair Utilizes a Novel - AWS
2015 · Cité 5 fois — cassette into the BAC followed by gap repair (B) Consecutive route 2; first gap repair
pdf P[acman]: A BAC Transgenic Platform for Targeted - AAAS
artificial chromosome (BAC) recombineering and ?C31-mediated transgenesis The BAC is maintained at low-copy number facilitating plasmid maintenance and recombineering but induced to high-copy number for plasmid isolation Recombineering allows gap repair and mutagenesis in bacteria Gap repair efficiently retrieves
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