[PDF] DIG RNA Labeling Mix, 10 × conc - Sigma-Aldrich
DIG-labeled, single-stranded RNA probes of defined length are generated by in vitro transcription DIG-11- UTP is incorporated by SP6, T7 and T3 RNA poly-
[PDF] DIG RNA Labeling Kit (SP6/T7) - Sigma-Aldrich
Transcription of control DNA 1 by T7 RNA polymerase according to the standard pro- tocol produces DIG-labeled “antisense” Neo transcripts of 760 bases in
[PDF] I In situ hybridization with DIG-labeled probes - Roche Life Science
RNA-RNA in situ hybridization using DIG-labeled probes: the effect of high molecular weight polyvinyl alcohol on the alkaline phosphatase indoxyl-nitroblue
[PDF] DIG RNA Probe Synthesis and Purification Lili Jing - Bio-protocol
this protocol is a detailed description of synthesizing an antisense DIG-labeled RNA probe for in situ hybridization using the DIG RNA labeling kit from Roche
[PDF] Making Dig labeled RNA Probes
Making Dig labeled RNA Probes 1) Amplify DNA via pcr, using plasmid primers ( ie T7,T3) 2) Put on gloves to protect the RNA you will make from the RNAse on
[PDF] HighYield T7 Digoxigenin RNA Labeling Kit - Jena Bioscience
DIG-11-UTP is efficiently incorporated into RNA as substitute for its natural counterpart UTP using an optimized reaction buffer and T7 RNA Labeling Polymerase
[PDF] A Method for High Quality Digoxigenin-Labeled RNA Probes for In
DIG-labeled RNA antisense probes are easily produced using PCR and in vitro transcription, avoiding tedious cloning steps In this note, we describe a method
[PDF] Title A Large-Scale Whole-Mount in situ Hybridization - CORE
A DIG-labeled RNA probe for WISH was synthesized from a PCR-amplified template which contained an RNA promoter All reactions of PCR and subsequent
[PDF] dig southern blot protocol
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