Lateral resolution enhancement with standing evanescent waves
the lateral resolution Dr of conventional microscopy is given approximately by Dr l e 2NA l e 2, where l e is the emission wavelength and NA is the numerical aperture of the imaging optics Many techniques such as confocal and 4 pi microscopy have attained Dr l e 3 (Ref 1; p 48, Ref 2) Addition-ally, atomic scale resolution has been achieved in
Study of spatial lateral resolution in off-axis digital
The lateral resolution in digital holographic microscopy (DHM) has been widely studied in terms of both recording and reconstruction parameters Although it is understood that once the digital hologram is recorded the physical resolution is fixed according to the diffraction theory and the pixel density, still
Lecture 10: Basics of Atomic Force Microscope (AFM)
Lateral Force Microscopy (LFM) 2µm x 2µm section on a Ni CD-stamper matrix The friction image presents a more detailed surface structure than the topography image Bright areas correspond to higher friction Topography Friction image T Göddenhenrich, S Müller and C Heiden, Rev Sci Instrum 65, (1994) 2870
CONFOCAL LASER SCANNING MICROSCOPY TUTORIAL
resolution) remains constant 2) For pinhole diameters greater than one Airy unit, the Z resolution slowly becomes worse 3) A pinhole diameter approximately 1/5 Airy unit will produce maximum lateral resolution at the expense of a loss of 95 of the signal 4) Lateral resolution is more sensitive to pinhole size than axial resolution and
Axial resolution of confocal Raman microscopes: Gaussian beam
considered to be confocal The lateral resolution is influenced directly by the fieldstop, which truncates the field of view for the detector In a confocal microscope the axial resolution is determined by the reduction of the out-of-focus signal The choice of a slit or pinhole entrance of the detector stage depends on the resolution and signal
Self-interference 3D super-resolution microscopy for deep
lateral localization precision was comparable for the two approaches, SELFI showed substantially better axial localization precision We then quantified the localization precision and accuracy for deep super-resolution imaging (Fig 2b,c) To mimic tissue studies, we acquired images at various depths in a phantom composed of
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