Northern Blot (sRNA) -- LICOR General Notes: Practice RNase
sRNA Northern Blot Protocol 6/24/10 (LMM) Revised 4/10/14 (LIU) Page 1 of 3 Northern Blot (sRNA) -- LICOR General Notes: Practice RNase-Free technique: -Use RNA-only pipet tips/tubes -Use RNaseZap to clean counters and pipetmen -Filter/sterilize solutions -Use DEPC-H 2O or MQ-H 2O -Keep RNA cold (4 ˚C or lower) Needed Material:
Northern Blot with Nitrocellulose
Northern Blot with Nitrocellulose 1 During the run of the glyoxal gel, measure gel exactly and set up for transfer to nitrocellulose (step 2) After run, cut off bottom right corner of gel The gel may be transferred immediately after electrophoresis is complete It is not necessary to place the glyoxal gel in 20X SSC before transfer
Department of Agronomy Northern Blotting
6 Pre-soak the gel in 20x SSC while setting up the Northern blot transfer (about 15 minutes) a Northern membrane blotting Northern membrane transfer is the same as described as in the Southern protocol with the following exceptions: 1 Use 20 x SSPE instead of 20 x SSC for transfer 2 All of the vessels need to be treated for removing RNase 3
SHORT REPORT Open Access A non-radioactive method for small
northern blot applications with this strategy, and this amount can easily be obtained by researchers without any special or expensive equipment Protocol for northern blotting with biotin-labeled probes The methodology described in this study is illustrated in Figure 4 First, the integrity of the RNAs is determined, and the total amount is
Detection of microRNAs by Northern blot analyses using LNA probes
cules In Northern blot hybridization assays, LNA-modi-fied oligonucleotide probes detected mature miRNAs by at least a 10-fold higher efficiency than traditional DNA probes [1] Moreover, LNA-modified oligonucleotide probes were highly specific, as demonstrated by the use of different single- and double-mismatched LNA probe molecules
Improved northern blot method for enhanced detection of small RNA
Improved northern blot method for enhanced detection of small RNA Gurman S Pall & Andrew J Hamilton Division of Cancer Sciences and Molecular Pathology, Faculty of Medicine, University of Glasgow
Développement dune méthode de quantification des ARN mess
3 Northern Blot Les mesures d'ARNm par Northern Blot ont ~t~ r~alis~es comme pr~c6demment d~crit [2] Les ARN totaux oat ~t~ extraits par la m~thode de Chomczynski et Sacchi modifi~e [11], ~ partir des cellules en cultu- re pour la lign~e HepG2 et ~ partir de frag- ments de parenchyme testiculaire de taille
Indolic uremic solutes increase tissue factor production in
Méthode : rappel Pour estimer l’expression d’un gène : ARN : Northern Blot quantitatif, short/long nucleotide array Protéine : Western Blot quantitatif,
Chapitre 1 La cellule : unité morphologique et fonctionnelle
8 La technique du southern-blot : d a FAUX C’est le western blot b FAUX Les ARNs sont détectées par une méthode de Northern-blot c FAUX d VRAI Le southern blot permet la détection d’un ADN 9 La technique du northern-blot : c, d a FAUX b FAUX c VRAI Le northern-blot permet la détection des ARNs 10 La technique ELISA : c
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