Stability of VACUETTE Lithium Heparin Separator tubes with
The anticoagulant heparin activates antithrombins, thus blocking the coagulation cascade and producing a whole blood/plasma sample making it ideal for rapid analysis and analysis of blood from patients on anticoagulant therapy VACUETTE® Lithium Heparin Separator tubes contain a barrier gel in the tube The specific gravity of this
USE OF ANTICOAGULANTS IN DIAGNOSTIC LABORATORY INVESTIGATIONS
Calcium-titrated heparin at a concentration of 40 to 60 IU/mL blood (dry heparinisation) and 8 to 12 IU/mL blood (liquid heparinisation) is recommended for the determination of ionized calc ium (22) 1 1 4 4 Hirudin Hirudin is an antithrombin extracted from leeches or prepared by a genetic engineering process
ISBER BIOSPECIMEN SCIENCE LITERATURE COMPILATION
Jensen EA, Stahl M, Brandslund I & Grinsted Stability of heparin blood samples during transport based on defined pre-analytical quality goals Clin Chem Lab Med 2008; 46(2): 225-234 Banks RE Preanalytical influences in clinical proteomic studies: Raising awareness of fundamental issues in sample banking Clinical Chemistry 2008; 54(1): 6-7
Coagulation Testing Sample Suitability and Rejection Criteria
9 parts blood to 1 part anticoagulant • Under-filled tubes - prolonged clotting times • Prolongation may be reagent dependent • < 90 fill is unacceptable unless locally validated • More forgiving with 3 2 vs3 8 sodium citrate* • Small volume tubes are less forgiving** • Samples with hematocrit > 55 require adjustment of citrate
“Do’s & Don’ts” for Reliable Specimen Quality
Red cells (RBCs) often have a much higher concentration of analytes than the liquid portion (serum/plasma) of blood For example, RBCs have 20x more potassium (K) than serum/plasma, so prolonged contact and/or contamination with red cells will significantly change K levels The practices below help ensure acceptable samples for all analytes
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