Elimination of Mycoplasma Contamination in Cell Cultures
remained infected It is concluded that eukaryotic cell cultures containing mycoplasmas are amenable to antibiotic treatment and that a cure rate of about three-quarters is a reasonable expectation Introduction Mycoplasma contamination of cell culture systems continues to present major prob-
Prevention and Detection of Mycoplasma Contamination in Cell
contamination; 5 the consequences of mycoplasma contamination in cell culture and 6 available methods to eliminate mycoplasma contamination Awareness about the sources of mycoplasma and pursuing aseptic techniques in cell culture along with reliable detec-tion methods of mycoplasma contamination can provide an appropriate situation to pre-
Prevention and Control of Mycoplasma Contamination in Cell
Effects of Mycoplasma Contamination Mycoplasma infected cell cultures should not be used for experimental or diagnostic work Figure 1 shows the results from two cultural tests for mycoplasma detection where a direct inoculation of the cell culture suspended in its normal medium has yielded confluent growth of mycoplasmas Such
Detection of Mycoplasma in cell cultures
Apr 22, 2010 · Mycoplasma contamination of cell cultures is widespread, ranging from 5 to 35 in published reports1 The use of contaminated cells compromises almost all aspects of cell physiology, and consequently the results and conclusions from any experiment2,3 It is essential that all cell stocks and all new cultures entering a laboratory are tested for
Detection Mycoplasma Contamination Cell Cultures by
tures (52 mycoplasma-positive and 43 mycoplasma-negative cell cultures) Moderate to high numbers of mycoplasmas (104 to 108 cells per ml) wereisolated from the 52 mycoplas-ma-positive cell cultures Conflicting results were obtained with nine cell cultures The microbiological culture results for mycoplasmas were negative for four cell
InvivoGen Review Mycoplasma Contamination of Cell Cultures
Mycoplasma contamination of cell cultures has been known for decades[1] and disturbingly, has become widespread, threatening academic labs to biopharmaceutical production facilities [2] In fact, depending on the laboratory, anywhere from
Detection of Mycoplasma Contamination of Cell Culture by A
Mycoplasma contamination of cell cultures To the best of our knowledge, this is the first report on application of this method for detection of Mycoplasma contamination
Animal Cell Cultures: Risk Assessment and Biosafety
than 96 of mycoplasma contaminants in cell cultures Primary sources of contamination with M orale, M fer-mentans, and M hominis in the laboratory are infected people who handle cell cultures and suspensions of vi-ruses Sources of M argini, and M hyorhinis are usually animal donors of tissues and biological constituents used
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