Evaluation of the Value of Frozen Tissue Section Used as “Gold
Key Words: Immunohistochemistry; Antigen retrieval; Frozen tissue section; Formalin fixation; Paraffin embedding. DOI: 10.1309/7CXUYXT23E5AL8KQ. Abstract.
Application of Heat-induced Antigen Retrieval to Aldehyde-fixed
SUMMARY. We applied the heat-induced antigen retrieval (HIAR) to aldehyde-fixed fresh frozen sections based on a new approach (i.e. a rapid and complete
Antigen Retrieval by Heating En Bloc for Pre-fixed Frozen Material
SUMMARY Antigen retrieval (AR) is frequently required for successful immunohistochem- istry (IHC) in archival formalin-fixed paraffin-embedded tissue
Immunohistochemistry (IHC-FR)-Frozen sections protocol
The absence of a formaldehyde based fixative eliminates the need for an antigen retrieval step. However if frozen tissue or cytological specimens have been
Protocol: Immunohistochemistry staining of frozen sections (IHC-Fr)
The optimal method of antigen retrieval must be determined experimentally. • Surround each tissue section with a hydrophobic barrier using a marking pen (
Protocol: Immunohistochemistry staining of frozen sections (IHC-Fr)
The optimal method of antigen retrieval must be determined experimentally . •. •. Surround each tissue section with a hydrophobic barrier using a marking pen (
Antigen retrieval technique utilizing citrate buffer or urea solution for
Human prostate tissues were immediately processed after surgical removal to obtain frozen tissue sections or paraffin-embedded sections. Frozen Sections. Fresh
Antigen Retrieval on Ultrathin Cryosections
We have explored antigen retrieval in cultured cells and in conventional cryostat sections of tissue fixed in paraformal- dehyde. We have shown that sodium
Tissue preparation and cryopreservation with sucrose -- for frozen
16 déc. 2016 Common cryoprotectants used to preserve tissue morphology include sucrose ... sections and they do not tolerate harsh antigen retrieval.
Antigen Retrieval Immunohistochemistry: Past Present
https://journals.sagepub.com/doi/pdf/10.1177/002215549704500301?download=true
