Acetone fixation will also permeabilize. Page 2. www.abcam.com/technical. 2. Methanol fixation can be used to permeablize but is not always suitable. These
formol-calcium fixation leaves sufficient enzyme activity in the brush border the reaction product is not as well lOcalize(las after acetone fixation.
Smears or cytospins : air dried. Fixation: Cold acetone (4°C) 10 min. Ethanol (not suitable for some antigens ex: ER
Acetone will also permeabilize. No further permeabilization step is required. Fixation method for tissue samples. Immersion fixation. 10% neutral buffered
of cold acetone fixation and formalin fixation for the detection of carcinoembryonic antigen (CEA) and keratin in sections from a variety of normal and
MeOH/Acetone fixation and staining of C. elegans for anti-PGL-1 whole-mount staining adapted by Erik Andersen from Mello Lab protocol by Daryl Conte
Mast cells in the nasal mucosa can be studied by means of monoclonal antibodies (mAb) against tryptase (T^MC) and chymase (C^MC). Fixation with acetone
commonly used fixation reagents in histochemical and in situ hybridization procedures. However acetone at 100% and some mixtures of acetone and alcohols.
(HIV) and preserve its antigenicity for antibody detection by immunofluorescence in MOLT-4-T4 cells. Air-dried cell smears were fixed in cold acetone
Whereas acetone fixation inactivates the original enzyme by 30-35% alcohol destroys only 10-25% of the activity. In so far as alcohol is concerned