For example when sucrose
Plants synthesize two forms of starch amylose
hydrolysis of sucrose into single sugars is necessary before the sugars can be metabolized. The concentration of water in this experiment is large 55M
03-Jul-2018 enzyme purity and hydrolysis of sucrose and ?-glucans have been studied in detailed. Results obtained for a range of starch containing ...
Polysaccharides serve for the storage of energy (e.g. starch in plants and glycogen In this experiment
About 3-5 g each of Glucose Fructose
Benedict's qualitative solution is a test reagent that reacts positively with (simple) reducing sugars. All monosaccharides and most disaccharides are reducing
The hydrolysis of sucrose or amylose can be achieved by using a strong acid such as HCl or with the aid of biological catalysts (i e enzymes) Starch can form an intense brilliant dark blue or violet colored complex with iodine The straight chain component of starch (or amylose) gives a blue color while the branched
Jan 11 2012 · Hydrolysis of Sucrose 7 Add 0 5 mL of 3 M HCl to 5 mL of a 1 sucrose solution in a test tube Mix Heat and stir the mixture in a boiling water bath for 20 minutes (You may add deionized water to this solution if the volume starts getting low!) Cool the solution and add 1 M NaOH until the solution tests neutral on litmus paper (To
EXPERIMENT 6 Properties of Carbohydrates: Solubility Reactivity Chirality and Specific Rotation Materials Needed About 3-5 g each of Glucose Fructose Maltose Sucrose Starch sodium bicarbonate NaHCO3(s) 15 mL 5 sucrose 25 mL 1 cooked starch suspension 5 mL 6 M HCl 50 mL Benedict's reagent 5 mL dilute iodine solution
3 Sucrose 4 Lactose 5 Starch Hydrolysis of Carbohydrates Hydrolysis of Sucrose (Acid versus Base Catalysis) Sample Condition of Hydrolysis Color Observation Fehling’s Test (positive or negative) 1 Acidic (H 2 SO 4) 2 Basic (NaOH) Acid-Catalyzed Hydrolysis of Starch Sample Heating Time (min) Color Observation Iodine Test (positive or negative)
Hydrolysis of Starch On a spot plate place 1 drop of dilute iodine solution in each depression In a large test tube place 20 mL of 1 cooked starch suspension Heat in a boiling water bath and when hot add 2 mL of 6 M hydrochloric acid (HCl)
Hydrolyzed starch and iodine solution Questions 1 According to the results of each part of the experiment identify your unknown and explain your reasoning Experiment 11 Report Page 5 2 Compare the results you obtained for the Fehling’s test of sucrose to the Fehling’s test of hydrolyzed sucrose Explain your results 3