We have designed an unique reaction system to analyze hydrolysis rate of phosphodiester linkage at elevated temperatures using oligonucleotides with sequence of
RNA RNA Degradation
isocratic elu tion with 10 m M.
probe to assign the rate-limiting step in RNA hydrolysis. A novel mechanism involving general acid catalysis by CoIII- bound water is proposed on the basis
15 Jun 2010 The active center of RNA polymerase can hydrolyze phospho- ... effects on the rate of second phosphodiester bond hydrolysis.
25 May 2021 Comparing the rate constant for. dsRNA hydrolysis to that for ssRNA hydrolysis indicates that. dsRNA was more resistant to alkaline hydrolysis ...
From the uncatalysed rates of UpA cleavage [23] and dimethyl phosphate (as a model for DNA) hydrolysis [24] we estimate the rate at which RNA depolymerizes in
24 Aug 2020 reduce mRNA hydrolysis is to redesign RNAs to form double-stranded regions ... model that links an RNA molecule's overall hydrolysis rate to ...
probe to assign the rate-limiting step in RNA hydrolysis. A novel mechanism involving general acid catalysis by CoIII- bound water is proposed on the basis
buffers (3) the rate of RNA breakdown rate of loss of intact 7.5-kb RNA was determined
Numerous methods have been utilized to follow the rate of the enzy- matic hydrolysis of RNA.' Bain and Rusch (1) measured the liberation.
Figure 2The TL is dispensable for intrinsic RNA hydrolysis on a locked scaffold (a) Nucleic-acid scaffold DNA and RNA are shown in black and red respectively Red asterisk 32P label (b)
RNAhydrolysis was detectable with 3 91 nM RNase A (Fig 3c)indicating a sensitivity of *5 ng (100ll of 3 91 nMRNase A corresponds to 5 4 ng of protein) This methodseems to be*400-fold more sensitive than the traditionalin situ gel electrophoresis-based method [13] whichrequires*2lg of protein
human systems By examining the basic thermodynamic equations for RNA interactions in RNAi we demonstrate how the free energies of RNA folding and phosphoester bond hydrolysis can drive RNAi without ATP Our calculations of RNAi efficiency are close to actual values obtained from in vitro experimental data from two previous studies for
To determine whether the rate of RNA binding limited the observed rate of hydrolysis of the RNA substrate we performed a rapid quench experiment with the dsRNA substrate using two