Meyerhof and his collaborators (3) studied the production of lactic acid in digests of glycogen with ground frog muscle or muscle extracts and Lohmann (4) has
ENZYMATIC HYDROLYSIS OF GLYCOGEN AND DETERMINATION OF. GLUCOSE . When liver is ground up with trichloroacetic acid (TCA) the larger molecules.
without extraction and acid hydrolysis of tissue glycogen. Homogenates were treated with amyloglucosidase which degrades glycogen to glucose
index detection for the determination of glycogen in sea urchin measuring glycogen either by acid hydrolysis or by enzymatic hydrolysis (5).
acid gives a quantitative yield of glucose. The specific optical Glock (2) has shown that the rate of enzymatic hydrolysis of glycogen is much slower t ...
For the enzymatic hydrolysis 100 mg. per cent solutions of poly- cent hydrolysis for acid-treated amylose and Schardinger dextrins are.
Results 20 - 30 precipitation of glycogen. In their method the tissue (liver or muscle) is first hydrolyzed .with KOH then autoclaved with acid;.
by acid hydrolysis of glycogen. The conditions were chosen to obtain a liberation of reducing power similar to that obtained by enzymatic hydrolysis in the
suring total muscle glycogen (Glytot) either by acid hydrolysis. (AC) or by enzymatic hydrolysis (EZ). As well
acids. Cell ultra-structure and cell metabolism were integrated with Acid and Enzymatic Hydrolysis of Glycogen: HCI and a-amylase hydrolysis:.