Single molecule biophysics lab

  • What is biophysics of single molecules?

    Single-molecule biophysics is the study of the dynamics and interactions of individual biomolecules to understand how they carry out their functions in living cells.
    For example, monitoring the folding properties of single protein or RNA molecules helps reveal how they are transported across cellular membranes..

  • What is single molecule biophysics techniques?

    Single-molecule biophysics is the study of the dynamics and interactions of individual biomolecules to understand how they carry out their functions in living cells.
    For example, monitoring the folding properties of single protein or RNA molecules helps reveal how they are transported across cellular membranes..

  • What is single molecule detection?

    Single molecule detection is a way to study detailed physical and chemical properties that allows for scrutiny of fundamental principles and mechanisms, and may lead to technological and methodological developments.
    Single molecule techniques also have key potential in material development..

  • What is single molecule science?

    Single Molecule Science—the ability to observe and track individual molecules and monitor molecular interactions in living cells—heralds a revolution in biology..

  • What is the biophysics of molecules?

    Molecular biophysics is the study of the physical principles governing biomolecular systems.
    It seeks to explain biological function in terms of molecular structure, dynamics and organization, from single molecules to supramolecular structures..

  • What is the principle of single molecule spectroscopy?

    Single-molecule spectroscopy (SMS) allows exactly one molecule hidden deep within a crystal, polymer, or cell to be observed via optical excitation of the molecule of interest (Fig. 1a)..

  • Why do we study single molecules?

    Single molecule methods can provide detailed information about molecular mechanisms and interactions, complementing bulk assays with additional information that is often difficult or impossible to observe using traditional methods..

  • Why single molecule biophysics?

    Single molecule methods can provide detailed information about molecular mechanisms and interactions, complementing bulk assays with additional information that is often difficult or impossible to observe using traditional methods..

  • Single molecule methods can provide detailed information about molecular mechanisms and interactions, complementing bulk assays with additional information that is often difficult or impossible to observe using traditional methods.
  • Single-molecule fluorescence spectroscopy is currently the most commonly used single-molecule technique.
    The basis of single-molecule fluorescence microscopy is the labeling of the molecules of interest with a fluorophore and observing it in an optical microscope.
  • The Molecular Level
    Molecular biophysics, an outgrowth of molecular biology, explores the structure of such biologically important macromolecules as nucleic acids, muscle proteins, enzymes, visual pigments in retinal cells, and lipoproteins in cell membranes.
First, molecular properties are measured one molecule at a time in a single-molecule experiment. Hence, in contrast with conventional ensemble  AbstractIntroductionSingle-molecule methods primer
In contrast to many ensemble-based experiments, single-molecule experiments on biological objects can be performed under biologically relevant conditions and, more importantly, can reveal information about dynamic or static heterogeneity and the possible existence of sub‐populations.
In the Laboratory of Single Molecule Biophysics, led by Dr. Keir C. Neuman, techniques---including optical and magnetic tweezers and fluorescence imaging, 
We aim to visualize the dynamic progressions of ensemble DNA mismatch repair (MMR) in vitro and in vivo to detail the mechanism of MMR using single-molecule 
We develop and combine cutting-edge single molecule and single cell techniques to solve important problems in biological science, including the organization 
We use state-of-the-art single-molecule techniques to probe the structure, dynamics, and interactions of biomolecules in living cells. Our research is driven by 

What are the methods of single-molecule manipulation and detection?

Our laboratory develops and applies novel methods of single-molecule manipulation and detection, such as:

  • optical tweezers
  • single-molecule fluorescence microscopy
  • ‘fleezers’ (fluorescence-force hybrid instrument)
  • and nanopore.

    • What is single molecule biophysics?

    In the Laboratory of Single Molecule Biophysics, led by Dr.
    Keir C.
    Neuman, techniques---including:

  • optical and magnetic tweezers and fluorescence imaging
  • in combination with conventional molecular biology approaches---are employed to answer fundamental questions concerning enzyme function and regulation.
    Enzymes are typically studied in ensembles.

    • What is the theoretical molecular biophysics laboratory?

    The Theoretical Molecular Biophysics Laboratory, led by Dr.
    Jose Faraldo-Gomez, investigates how the biological activity of membrane proteins emerges from their structure, dynamics and environment.

    Single-molecule fluorescence resonance energy transfer is a biophysical technique used to measure distances at the 1-10 nanometer scale in single molecules, typically biomolecules.
    It is an application of FRET wherein a pair of donor and acceptor fluorophores are excited and detected at a single molecule level.
    In contrast to ensemble FRET which provides the FRET signal of a high number of molecules, single-molecule FRET is able to resolve the FRET signal of each individual molecule.
    The variation of the smFRET signal is useful to reveal kinetic information that an ensemble measurement cannot provide, especially when the system is under equilibrium with no ensemble/bulk signal change.
    Heterogeneity among different molecules can also be observed.
    This method has been applied in many measurements of intramolecular dynamics such as DNA/RNA/protein folding/unfolding and other conformational changes, and intermolecular dynamics such as reaction, binding, adsorption, and desorption that are particularly useful in chemical sensing, bioassays, and biosensing.

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