Laboratory safety
Similarly, other organisations classify B. pertussis as a Biosafety Level-2 (BSL-2) pathogen or belonging to Risk Group 2. Thus, handling these agents is carried out at BSL-2 using a class I microbiological safety cabinet for potentially aerosol-generating procedures.
pertussis as a Biosafety Level-2 (BSL-2) pathogen or belonging to Risk Group 2.
Similarly, other organisations classify B. pertussis as a Biosafety Level-2 (BSL-2) pathogen or belonging to Risk Group 2.
Is Bordetella parapertussis a virulence factor?
Bordetella parapertussis can cause a milder form of pertussis.
The bordetellae are small, Gram-negative, aerobic coccobacilli.
Bordetella pertussis produces a number of virulence factors, including:
pertussis toxin adenylate cyclase toxin filamentous hemagglutinin and hemolysin. Is pertactin required for Bordetella species to resist neutrophil-mediated clearance?
Pertactin is required for Bordetella species to resist neutrophil-mediated clearance:2901–2909. doi:
10.1128/IAI.00188-10.
Khelef N, Bachelet CM, Vargaftig BB, Guiso N. 1994.
Characterization of murine lung inflammation after infection with parental Bordetella pertussis and mutants deficient in adhesins or toxins:2893–2900. Section II - Hazard Identification
PATHOGENICITY/TOXICITY: B. pertussis is a respiratory pathogen that causes pertussis, also known as whooping cough, a localized infection of the ciliated epithelium of the bronchial tree Footnote 3Footnote.
7) The disease affects mainly children, but adults have also been increasingly reported to be affected Footnote 2Footnote.
8) The pathogen produc.
Section III - Dissemination
RESERVOIR: Humans are the sole reservoir Footnote.
3) The main reservoir consists of adults and adolescents with atypical or undiagnosed infection, who may transmit the infection to infants and children Footnote 8. ZOONOSIS: None Footnote.
3) VECTOR: None Footnote 3.
Section IV – Stability and Viability
DRUG SUSCEPTIBILITY/RESISTANCE: Susceptible to erythromycin, macrolides such as azithromycin and clarithromycin, trimethoprim-sulfamethoxazole, floroquinoles such as ciprofloxacin, levofloxacin, and gemifloxacin Footnote 3Footnote 12.
DRUG RESISTANCE: Erythromycin resistant strains have been reported since 1994, but the resistance does not seem to .
Section V – First Aid / Medical
SURVEILLANCE: Monitor for symptoms.
Diagnosis of B. pertussis can be done using cultures of clinical specimens such as nasopharygeal aspirates and posterior nasopharyngeal swabs Footnote 2Footnote 3, however, it should be noted that cultures are less sensitive after antimicrobial therapy has been started Footnote.
2) Gram-staining or biochemical tes.
Section Vi - Laboratory Hazards
LABORATORY-ACQUIRED INFECTIONS: Eight cases of B. pertussis infection were reported from a research institution conducting pertussis research Footnote.
8) The infections were found among individuals not directly working with the bacteria.
They may have acquired the infection through the common laboratory spaces where the research was conducted Footn.
Section VII – Exposure Controls / Personal Protection
RISK GROUP CLASSIFICATION: Risk Group 2 Footnote 22.
CONTAINMENT REQUIREMENTS: Containment Level 2 facilities, equipment, and operational practices for work involving infectious or potentially infectious materials, animals, or cultures Footnote 23.
PROTECTIVE CLOTHING: Lab coat.
Gloves when direct skin contact with infected materials or animals is .
Section VIII – Handling and Storage
SPILLS: Allow aerosols to settle and, wearing protective clothing, gently cover spill with paper towels and apply an appropriate disinfectant, starting at the perimeter and working towards the centre.
Allow sufficient contact time before clean up Footnote 23.
DISPOSAL: Decontaminate all wastes that contain or have come in contact with the infectiou.
What agar is used to grow Bordetella pertussis?
For routine use, charcoal-blood agar (REGAN-LOWE medium) is most widely used.
A (2,6-O-dimethyl)-b-cyclodextrin supplemented STAINER-SCHOLTE broth can be used as an enrichment medium.
The Bordetella species do not need factors X and V (NAD + and hemin).
Bordetella pertussis usually grows after 3 to 4 days of incubation at 37 C.
What is Bordetella pertussis?
SYNONYM OR CROSS REFERENCE:
Originally named Haemophilus pertussis F 1 Whooping cough 2.
CHARACTERISTICS:Bordetella pertussis are small, gram-negative, encapsulated, non-motile, coccobacilli with outer pili.
They are generally about 0.5-1.0 µm in size 3 - 5. 