Decontamination and Cleaning One method is to use a 1:10 fresh bleach solution followed by a 70% ethanol rinse to avoid corrosion and achieve good disinfection. Contact EHS if you need a bleach alternative. Reaching the back and sides of the biosafety cabinet can be difficult..
How should vacuum lines be protected?
The vacuum system should be protected by a HEPA filter, particularly when used to manipulate biohazardous materials/infectious waste, and an overflow container should be present and managed vigilantly..
What pressure does a biosafety cabinet has?
These cabinets should be maintained under a minimum negative pressure of 0.5" w.g. Exhaust air is discharged to the outdoors through double HEPA filters (or HEPA and air incineration)..
Biosafety cabinets also provide HEPA-filtered air to the work area for product protection, plus air passes through a HEPA filter before being exhausted from the cabinet for environmental protection.
Paraformaldehyde is heated in a pan over a burner inside the BSC chamber to create formaldehyde gas, which is circulated through the BSC for 6-12 hours. After the formaldehyde contact time, the formaldehyde gas must be neutralized.
The vacuum system should be protected by a HEPA filter, particularly when used to manipulate biohazardous materials/infectious waste, and an overflow container should be present and managed vigilantly.
Vacuum systems must be protected with liquid disinfectant traps. In-line High Efficiency Particulate Air (HEPA) filters are recommended for Biosafety Level 2 work. These prevent hazardous biological materials from entering the vacuum lines.
A list of components including inline HEPA Filter, Flasks, Tubing and Stoppers; The “Dos” and “Don'ts” of assembling a
The Basic Design of a functional vacuum trap for your BSC; Recommendations regarding how to assemble a vacuum trap; A list of components
Housing the trap(s) entirely within a biosafety cabinet (BSC) or fume hood will significantly reduce the risk of exposure to potentially infectious aerosols and hazardous fumes, respectively.
Assembly of A Vacuum Flask System
Equipment Needed:. 1) Two vacuum flasks, preferably plastic to avoid breakage. 2) Thick-walled plastic tubing. 3) Glass or plastic tubes. 4) Rubber Stoppers. 5) Filters:
Disinfectant
Fill the Aspiration Vacuum Flask with a disinfectant. If using bleach, fill to about 10% of the flask’s volume. Keep in mind that when bleach and water are mixed together, the disinfectant qualities of bleach will degrade after 24 hours. You will need to add additional bleach prior to disposal. Do not use alcohol due to flammability and disposal co.
Set-Up
Aspirator bottles or suction flasks should be connected to overflow collection flasks containing an appropriate disinfectant ( A and B) and to an in-line HEPA (C) or equivalent filter before the vacuum line (D). Note that the glass or plastic tube should be placed into the solution in flask B to minimize aerosols. If the flasks are located outside .
What is Biosafety Level 2?
The CDC/NIH Biosafety In Microbiological and Biomedical Laboratories, 5 th ed., contains recommendations regarding protection of vacuum systems when working with biosafety level 2 materials. The combination of disinfectant-filled flasks and an in-line filter protects personnel who service the equipment and the vacuum system from contamination.
Biosafety cabinet vacuum
Insulated storage vessel
A vacuum flask is an insulating storage vessel that greatly lengthens the time over which its contents remain hotter or cooler than the flask's surroundings. Invented by Sir James Dewar in 1892, the vacuum flask consists of two extiw>flasks, placed one within the other and joined at the neck. The gap between the two flasks is partially evacuated of air, creating a near-vacuum which significantly reduces heat transfer by conduction or convection. When used to hold cold liquids, this also virtually eliminates condensation on the outside of the flask.
