Hepg2 biosafety level

  • Is HepG2 a HCC or HB?

    HepG2 is a hepatoblastoma cell line.
    It is used in a wide range of studies: a model of hepatoblastoma (HB) and hepatocellular carcinoma (HCC), cytotoxicity testing and the identification of drug metabolic pathways on the liver..

  • What are the properties of HepG2?

    HepG2 cells have adherent properties and grow as monolayers in small aggregates.
    HepG2 can be grown successfully at a large scale and stimulated with human growth hormone.
    They are also capable of secreting many plasma proteins, such as transferrin, fibrinogen, plasminogen and albumin..

  • What is HepG2?

    HepG2 cells are a hepatoblastoma cell line widely used in biological science research.
    Initially mistaken as hepatocellular carcinoma, they offer valuable insights into various aspects of liver-related studies..

  • What is the difference between HepG2 and Hep3B?

    Hep3B cells are commonly used in drug metabolism and hepatotoxicity studies.
    In contrast to HepG2 cells, Hep3B cells are hepatitis B virus-positive and tumorigenic.
    Hep3B cells have an average of 60 chromosomes per cell, while HepG2 cells contain an average of 55 (50? 56) chromosomes per cell..

  • Where are HepG2 cells located?

    Hep G2 (or HepG2) is a human liver cancer cell line..

  • Where do HepG2 cells come from?

    HepG2 was derived from a liver hepatocellular carcinoma of a 15 year old Caucasian male.
    The cells express 3-hydroxy-3-methylglutaryl-CoA reductase and hepatic triglyceride lipase activities..

  • Why do we use HepG2 cell line?

    HepG2 is a human hepatoma that is most commonly used in drug metabolism and hepatotoxicity studies.
    HepG2 cells are nontumorigenic cells with high proliferation rates and an epithelial-like morphology that perform many differentiated hepatic functions..

  • Hep G2 is an immortal cell line which was derived in 1975 from the liver tissue of a 15-year-old Caucasian male from Argentina with a well-differentiated hepatocellular carcinoma.
    These cells are epithelial in morphology, have a modal chromosome number of 55, and are not tumorigenic in nude mice.
  • HepG2 cells are a hepatoblastoma cell line widely used in biological science research.
    Initially mistaken as hepatocellular carcinoma, they offer valuable insights into various aspects of liver-related studies.
  • HepG2 cells have adherent properties and grow as monolayers in small aggregates.
    HepG2 can be grown successfully at a large scale and stimulated with human growth hormone.
    They are also capable of secreting many plasma proteins, such as transferrin, fibrinogen, plasminogen and albumin.
  • HepG2-Dual™ cells should not be passaged more than 20 times to remain fully efficient.
    HepG2-Dual™ cells should be maintained in growth medium supplemented with Blasticidin and Zeocin\xae.
  • The Hep-G2/2.2. 15 human hepatoblastoma cell line, a subclone of HepG2 human hepatoblastoma that stably expresses the hepatitis B virus, is one of the most widely used models for HBV-associated liver disease.
HepG2 is a human cell line derived from hepatocellular carcinoma from a 15 yr male. (1) These cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment.
HepG2 is a human cell line derived from hepatocellular carcinoma from a 15 yr male. (1) These cells are not known to harbor an agent recognized to cause disease in healthy adult humans. Handle as a potentially biohazardous material under at least Biosafety Level 1 containment.

Does HepG2 cell line heterogeneity allow in vitro biomedical studies?

In this regard, we set out to collect up-to-date information on the HepG2 cell line to assess whether the level of heterogeneity of the cell line allows in vitro biomedical studies as a model with guaranteed production and quality.
Keywords:

  • HepG2 cell line
  • mutations
  • hepatocellular carcinoma
  • hepatoblastoma
  • hepatocytes 1.
    Introduction .
  • How much DNA does a HepG2 cell contain?

    The HepG2 cell contains about 7.5 pg of DNA, 15% more than in a normal somatic cell [12,90].

    Is HepG2 a suitable model for metabolism-mediated toxicity?

    According to the article (Ren et al.), the HepG2 cell line may not be a suitable model in investigating metabolism-mediated toxicity without additional modification due to it is lack of metabolic capability [174,175].
    For example, the cell line can be used in studies of CYP inducers .

    What is Hep G2?

    Hep G2 [HEPG2] is a cell line exhibiting epithelial-like morphology that was isolated from a hepatocellular carcinoma of a 15-year-old, White, male youth with liver cancer.
    The cell line was deposited by the Wistar Institute and is a suitable transfection host.
    Expression markers include:

  • insulin; insulin-like growth factor II (IGF II).

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