Protein ladders are composed of a series of bands, which are highly purified polypeptides of different well-defined sizes.
These polypeptides get resolved during electrophoresis as separate bands - based on their molecular weights..
Unstained protein ladders offer more accurate protein sizing because the dyes in prestained ladders can slightly distort the proteins on the gel.
These ladders include easy-to-purify proteins of known molecular weight..
Protein ladders, also known as protein markers or protein standards, are used to help estimate the size of proteins separated during electrophoresis.
They serve as points of reference because they contain mixtures of highly purified proteins with known molecular weights and characteristics..
Protein ladders, also known as protein markers or protein standards, are used to help estimate the size of proteins separated during electrophoresis.
They serve as points of reference because they contain mixtures of highly purified proteins with known molecular weights and characteristics..
Fluorescent protein ladders are used in SDS-PAGE gels or polyacrylamide gel electrophoresis (involving tris-glycine buffer).
They contain a mixture of proteins that are pre-stained with fluorescent dyes.
The bands fluoresce when the light of a specific wavelength falls on them..
Protein ladders or molecular weight markers are among the most commonly used reagents in biochemistry experiments.
They provide molecular weight standards to estimate the size of proteins separated by gel electrophoresis like SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis)..
Western blotting ladders provide a useful positive control to evaluate the performance of the detection antibody.
Direct visualization of unstained ladders can also be achieved through standard staining protocols (e.g., Coomassie stains)..
Western blotting ladders provide a useful positive control to evaluate the performance of the detection antibody.
Direct visualization of unstained ladders can also be achieved through standard staining protocols (e.g., Coomassie stains)..
Protein ladders, also known as protein markers or protein standards, are used to help estimate the size of proteins separated during electrophoresis.
They serve as points of reference because they contain mixtures of highly purified proteins with known molecular weights and characteristics..
Because of being denatured and then coated in a negative charge, the proteins will migrate and separate based on size.
To determine the size of the proteins, a molecular weight marker, or protein ladder, is used.
Often, these ladders are pre-stained so you can visually detect the progress of the protein separation..
Increased overall brightness provides visual robustness throughout the entire protein electrophoresis and western blotting workflow.
Improved Dual Color Standards are brighter than the competition on both gels and blots.
Increased brightness makes it easier to:.
1) Identify target proteins on the gel or blot.
2) Monitor gel electrophoresis.
3) Confirm.
Protein Ladders and Standards
More Precision Plus Protein Standards:.
1) Precision Plus Protein Unstained Standards.
2) Precision Plus Protein Prestained Standards.
3) Precision Plus Protein™ WesternC™ Standards Standard plugs are offered for 2-D electrophoresis applications:.
1) Precision Plus Protein Unstained Standard Plugs, Strep-tagged, recombinant, pkg of 24 (1610378)
Precision Plus Protein Dual Color Standards Instruction Manual
Bio-Rad IEF standards are natural proteins with isoelectric points ranging from 4.45 to 9.6.
The 2-D SDS-PAGE standards have a pI range from 4.5 to 8.5 and a molecular weight range from 17.5 to 76 kD.
Bio-Rad protein ladders have been formulated and combined for use as molecular weight markers in a variety of applications including:
For 2-D SDS PAGE, the protein ladder must contain proteins that both span an appropriate pI range and, when reduced and SDS-denatured, cover a suitable molecular weight range.
Bio-Rad IEF standards are natural proteins with isoelectric points ranging from 4.45 to 9.6.
Every recombinant protein ladder is broad range.
Prestains and Tags:
