Computational methods nucleic acid

  • What are nucleic acids used for the coding for?

    ​Nucleic Acids
    Deoxyribonucleic acid, or DNA, encodes the information cells need to make proteins.
    A related type of nucleic acid, called ribonucleic acid (RNA), comes in different molecular forms that play multiple cellular roles, including protein synthesis..

  • What are the analytical technologies for nucleic acids?

    Nucleic acid quantification methods include measurement of fluorescent DNA or RNA binding dyes, measurement of absorbance at 260nm wavelength, and qPCR.
    Nucleic acid purity also provides a decision point to proceed with downstream assays or not based on 260/230 nm or 260/280 nm ratios..

  • What are the methods applied in nucleic acid analysis?

    Experimental methods fundamental to nucleic acid analysis include nucleic acid extraction and clean up, DNA/RNA quantification, PCR, molecular cloning and sequencing..

  • What are the methods for measuring nucleic acids?

    For the determination of nucleic acids from sediments, two methods are generally used: spectrophotometric, based on the specific absorbance of nucleic acids (9, 18), and fluorometric, using specific fluorescent stains (32, 45)..

  • What are the methods of nucleic acid analysis?

    Experimental methods fundamental to nucleic acid analysis include nucleic acid extraction and clean up, DNA/RNA quantification, PCR, molecular cloning and sequencing..

  • What are the methods of nucleic acid preparation?

    Phenol–chloroform extraction is widely used.
    The process consists of vigorous mixing of phenol–chloroform solution and sample followed by centrifugation [7].
    Phenol does not completely inhibit RNase activity, and this characteristic enables isolation of nucleic acid by combination with chloroform and alcohol [12]..

  • What are the methods of studying nucleic acid?

    These include PCR, RT PCR, sequencing and blotting.
    There are also techniques such as EMSA, footprinting, ChIP and probing that detect inter-molecular interactions of nucleic acids..

  • What technique is used to determine nucleic acid orders?

    Determining the order of the nucleotides within a gene is known as DNA sequencing.
    The earliest DNA sequencing methods were time consuming, but a major breakthrough came in 1975 with the development of the process called Sanger sequencing..

  • Why do we need to study nucleic acid?

    Nucleic acids are the most important macromolecules for the continuity of life.
    They carry the genetic blueprint of a cell and carry instructions for the functioning of the cell..

  • Bioinformatics has been conceptualizing biological process in terms of genomics and applying computer science (derived from disciplines such as applied modeling, data mining, machine learning and statistics) to extract knowledge from biological data.
  • Determining the order of the nucleotides within a gene is known as DNA sequencing.
    The earliest DNA sequencing methods were time consuming, but a major breakthrough came in 1975 with the development of the process called Sanger sequencing.
  • Nucleic acid analysis (genomics) involves isolation and characterization of DNA or RNA for use in applications such as genotyping, gene expression analysis, epigenetic analysis, microbiome studies and more.
  • Nucleic acids are biopolymers, macromolecules, essential to all known forms of life.
    They are composed of nucleotides, which are the monomer components: a 5-carbon sugar, a phosphate group and a nitrogenous base.
    The two main classes of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA).
Computational Biology / methods*; Databases, Protein; Gene Editing; Humans; Machine Learning; Molecular Conformation; Nucleic Acids / chemistry*; Protein 
Due to some limitations of experimental investigations, computational methods have played an important role in obtaining the knowledge of protein-nucleic acid 
Over the past few years, dozens of computational tools have been used for identification of nucleic acid binding site for site-specific proteins and design of 
Computational methods nucleic acid
Computational methods nucleic acid
Nucleic acid design is the process of generating a set of nucleic acid base sequences that will associate into a desired conformation.
Nucleic acid design is central to the fields of DNA nanotechnology and DNA computing.
It is necessary because there are many possible sequences of nucleic acid strands that will fold into a given secondary structure, but many of these sequences will have undesired additional interactions which must be avoided.
In addition, there are many tertiary structure considerations which affect the choice of a secondary structure for a given design.

Experimental process

Experimental approaches of determining the structure of nucleic acids, such as RNA and DNA, can be largely classified into biophysical and biochemical methods.
Biophysical methods use the fundamental physical properties of molecules for structure determination, including X-ray crystallography, NMR and cryo-EM.
Biochemical methods exploit the chemical properties of nucleic acids using specific reagents and conditions to assay the structure of nucleic acids.
Such methods may involve chemical probing with specific reagents, or rely on native or analogue chemistry.
Different experimental approaches have unique merits and are suitable for different experimental purposes.

Computational prediction of nucleic acid structure

Nucleic acid structure prediction is a computational method to determine secondary and tertiary nucleic acid structure from its sequence.
Secondary structure can be predicted from one or several nucleic acid sequences.
Tertiary structure can be predicted from the sequence, or by comparative modeling.

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