Computational methods for single-particle electron cryomicroscopy

  • How does cryo electron tomography work?

    During cryo-ET, a beam of electrons is passed through the sample as it is tilted in known increments about an axis.
    The individual projection images from the tomographic tilt series are then combined computationally in a procedure known as back-projection, which creates the .

    1. D tomographic volume

  • How does single particle cryo-EM work?

    In single particle analysis, purified proteins or protein complexes are suspended in amorphous (vitreous) ice through rapid plunge freezing, which preserve the samples' native structures.
    Transmission electron microscopy (TEM) is then used to collect numerous .

    1. D snapshots of the samples

  • What is the single particle approach?

    Single particle analysis is an increasingly popular cryo-electron microscopy (cryo-EM) technique that allows you to investigate biomolecules at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies..

  • During cryo-ET, a beam of electrons is passed through the sample as it is tilted in known increments about an axis.
    The individual projection images from the tomographic tilt series are then combined computationally in a procedure known as back-projection, which creates the .
    1. D tomographic volume
  • Single particle analysis is an increasingly popular cryo-electron microscopy (cryo-EM) technique that allows you to investigate biomolecules at near-atomic resolutions, unraveling dynamic biological processes and the structure of biomolecular complexes/assemblies.
It is an imaging method that does not require crystallization and can capture molecules in their native states. In single-particle cryo-EM, the 3D molecular structure needs to be determined from many noisy 2D tomographic projections of individual molecules, whose orientations and positions are unknown.
The computational pipeline in single-particle cryo-EM can be roughly split into two stages. The first stage consists of methods that operate at the level of entire micrographs such as motion correction, CTF estimation, and particle picking.

Method to determine atomic positions in solids using an electron microscope

Electron crystallography is a method to determine the arrangement of atoms in solids using a transmission electron microscope (TEM).
It can involve the use of high-resolution transmission electron microscopy images, electron diffraction patterns including convergent-beam electron diffraction or combinations of these.
It has been successful in determining some bulk structures, and also surface structures.
Two related methods are low-energy electron diffraction which has solved the structure of many surfaces, and reflection high-energy electron diffraction which is used to monitor surfaces often during growth.
Computational methods for single-particle electron cryomicroscopy
Computational methods for single-particle electron cryomicroscopy
Transmission electron cryomicroscopy (CryoTEM), commonly known as cryo-EM, is a form of cryogenic electron microscopy, more specifically a type of transmission electron microscopy (TEM) where the sample is studied at cryogenic temperatures.
Cryo-EM, specifically 3-dimensional electron microscopy (3DEM), is gaining popularity in structural biology.

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