Crystallography grade

How do you set up crystallization?
Increasing the concentration of a precipitating agent to a point just below supersaturation and then adjusting the pH or temperature to reduce the solubility of the protein can be used to crystallize samples with low protein concentration..
How long does it take to crystallize a protein?
Anything from 10 minutes to a year.
Lysozyme, for example, is an enzyme found in tear fluid.
It takes 10 minutes to grow lysozyme crystals.
A new protein may take a year because I have to work out the best conditions for growing crystals..
How much protein do you need for crystallography?
Crystallization typically requires 10 mg/mL concentration for proteins in the 10-30 kDa range.
Larger protein tend to require less concentration (2-5 mg/mL).
Smaller proteins tend to require higher concentration (20-50 mg/mL)..
Crystallizing Proteins at Small-Scale
Water from the drop containing the protein vaporizes and is attracted to the higher concentration solution in the reservoir.
As the concentration of solutes in the drop increases, protein begins to crystallize.

Dec 28, 2021The protein for crystallization should be homogeneous and >90% purity. The purity of protein shall be checked by HPLC or MS before

How do you set up crystallization?