X ray crystallography ppt download

How to do X-ray crystallography?
A purified sample at high concentration is crystallised and the crystals are exposed to an x ray beam.
The resulting diffraction patterns can then be processed, initially to yield information about the crystal packing symmetry and the size of the repeating unit that forms the crystal..
Is X-ray crystallography hard?
The most difficult part of the process is growing a perfect crystal, as this is needed to provide accurate information about the sample.
Some macromolecules, especially those with a high atomic weight such as membrane proteins, can be difficult to crystallize..
Techniques for Protein Analysis
X-ray crystallography is a method used for various materials in the crystallized state to determine the arrangement of atoms within a crystal.
Three-dimensional structure and function of many biological molecules, including proteins and nucleic acids, can be discovered by this method.
These include a source, a device to select and restrict the wavelengths used for measurement, a holder for the sample, a detector, and a signal converter and readout.
However, for x-ray diffraction; only a source, sample holder, and signal converter/readout are required.

How to do X-ray crystallography?