High throughput crystallography

  • How high throughput screening is done?

    Traditional HTS methods use solutions at a single concentration mostly at 10 μM whereas, in quantitative high throughput screening or qHTS approaches, compounds are tested at multiple concentrations using HTS platform and generate concentration-response curves for a large number of compounds in a single experiment..

  • What is a high throughput analysis?

    High-throughput (HT) analysis is becoming more and more important.
    It means analysis of dozens, hundreds, or even thousands of samples per day in a given laboratory or on a particular instrument..

  • What is high throughput crystallization?

    Our protein crystallization process is based on preparing blocks of crystal screens in 96-well deep well plates using a liquid handling robot, which is also used to transfer the same reagents, typically 100 microliters, to crystal plates as mother liquor..

  • What is meant by high throughput?

    High throughput screening (HTS) is the use of automated equipment to rapidly test thousands to millions of samples for biological activity at the model organism, cellular, pathway, or molecular level..

  • What is the high throughput method?

    Definition.
    High throughput screening (HTS) is the use of automated equipment to rapidly test thousands to millions of samples for biological activity at the model organism, cellular, pathway, or molecular level..

  • High-throughput (HT) analysis is becoming more and more important.
    It means analysis of dozens, hundreds, or even thousands of samples per day in a given laboratory or on a particular instrument.
  • High-throughput screening (HTS) is a key process used in drug discovery to identify hits from compound libraries that may become leads for medicinal chemistry optimization.
High-throughput crystallography is now applicable to screen molecular fragments that bind protein targets and define precisely their binding sites. It can subsequently be used as a rapid technique to guide lead optimisation.
Virtual screening coupled with high-throughput X-ray crystallography focuses on identifying one or more weakly binding small-molecule fragments from compound libraries that consist of hundreds of small-molecule fragment(s).

Can high-throughput crystallography help lead discovery?

High-throughput crystallography is now applicable to screen molecular fragments that bind protein targets and define precisely their binding sites

It can subsequently be used as a rapid technique to guide lead optimisation

In this review, we focus on high-throughput crystallography and its application to lead discovery

Is protein crystallography a truly HT method?

As with more established drug-discovery techniques, such as high-throughput screening (HTS), the key to further development of protein crystallography as a truly HT method is in improved efficiency, integration and automation 14

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Efforts are well underway to automate the entire structure-determination process

What is high-throughput crystallization of proteins?

Opin Struct Biol 10, 558563 (2000)

High-throughput crystallization of proteins has been advanced by exploiting techniques developed for the combinatorial chemistry industry, including robust liquid systems for handling and mixing small volumes

High-throughput crystallography is now applicable to screen molecular fragments that bind protein targets and define precisely their binding sites. It can subsequently be used as a rapid technique to guide lead optimisation. In this review, we focus on high-throughput crystallography and its application to lead discovery.

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