Hampton crystallography

  • How does hanging drop crystallization work?

    A drop composed of a mixture of sample and reagent is placed in vapor equilibration with a liquid reservoir of reagent.
    Typically the drop contains a lower reagent concentration than the reservoir.
    To achieve equilibrium, water vapor leaves the drop and eventually ends up in the res- ervoir..

  • What is Hampton Research?

    Hampton Research make and distribute products for protein crystallization..

  • What is Hampton Research?

    Hampton Research make and distribute products for protein crystallization.Jun 7, 2005.

  • Two of the most commonly used ground-based methods for protein crystallization fall under this category.
    These are known as the “hanging drop” and “sitting drop” methods.
  • Vapor diffusion is the most commonly employed method of protein crystallization.
    In this method, droplets containing purified protein, buffer, and precipitant are allowed to equilibrate with a larger reservoir containing similar buffers and precipitants in higher concentrations.
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for  Crystal Growth 101 LiteratureProductsCrystallization ScreensMake Tray
Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for  Crystal Growth 101 LiteratureProductsCrystallization ScreensContact UsLatest from hamptonresearch.comRapiData 2024 at SSRLHampton Research 3 days agoNew Additive Screen MiniHampton Research 1 week agoResearch Engineer PositionHampton Research 2 weeks agoSEA COAST 2024 29 Jan - 7 FebHampton Research 2 weeks agoPostdoctoral FellowHampton Research 1 month agoVapor Diffusion to MicrobatchHampton Research 1 month ago4th Generation SynchrotronHampton Research 1 month agoLego DNA 2.0Hampton Research 2 months agoSenior ScientistHampton Research 2 months agoWizard Screen now available!Hampton Research 2 months ago

How do you stop crystallization in a Hampton screen?

In some cases varying the pH by 0

1 pH unit is enough to stop crystallization In the Hampton screens the pH given (that of the buffer) is not the pH of the final solution Protein concentration The final protein concentration in the drop can be increased by increasing the ratio of protein to precipitant in the set-up Temperature

What is Hampton research Crystal screening?

Hampton Research Crystal Screen Based on the method published by Jancarik and Kim (Sparse matrix sampling: a screening method for crystallization of proteins

Jancarik, J and Kim, S-H J Appl Cryst

24:409-411, 1991) 10ml each of 50 conditions

Who is Hampton research?

Keep Me Posted Hampton Research, first in crystallization since 1991, developing and delivering crystallization and optimization screens, reagents, plates, and other tools for the crystallization of biological macromolecules, including proteins (antibody), peptides (insulin), and nucleic acids (DNA)


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