Crystallography soaking

How do you soak protein crystals?
The simplest way to soak a compound into a crystal is by adding either a concentrated stock solution or pure compound directly to the crystals in the crystallization drop.
This reduces crystal manipulation, especially for fragile crystals, and allows automation of the process (Krojer et al., 2017 ▸)..
What affects protein crystallization?
7.11.
Protein crystallization is the process of obtaining a protein crystal by addition of precipitating agents and controlling the conditions, like temperature, ionic strength, and pH of the solution..
What is crystal soaking?
The potential ligand is free to bind to the protein in solution prior to the formation of a crystal lattice, allowing the freedom for potential structural changes.
Crystal soaking is the process of taking pre-grown crystals and soaking them with the small molecule of interest..

Soaking for 10-30 min should be sufficient to populate the protein in the crystal if the binding site is accessible in the crystal lattice. If protein molecules

The major use of soaking is for the introduction of heavy atom substances into crystals for the determination of phases in the techniques of single (SIR) and multiple isomorphous replacement (MIR) which is needed for the determination of macromolecular crystal structures that lack a model suitable for molecular

We have found useful criteria to be using the peptide at >95% purity and performing the soaking at 277 K with an incubation time of one week. A peptide sample AbstractIntroductionResultsDiscussion and conclusions

Does Aege soaking affect crystallographic structure?

The soaking procedure did not induce changes in the crystals, which is reflected in a conserved overall molecular structure when comparing the AeGe-soaked structures with the published DPP8 unbound structure 6eoo (0

34 Å C α RMSD; Ross et al , 2018 )

The crystallographic structure of the AeGe-soaked DPP8–1G244 crystals was determined at 3

2 Å

What is Crystal soaking?

This method described as crystal soaking appears as a very promising approach to rapidly characterize fragment binding via a crystallographic strategy directly applied on protein crystals

However, also the soaking technique is not without problems

Why do macromolecular crystals need to be soaked?

Soaking of macromolecular crystals allows the formation of complexes via diffusion of molecules into a preformed crystal for structural analysis

Soaking offers various advantages over co-crystallization, e

g small samples and high-throughput experimentation

The major use of soaking is for the introduction of heavy atom substances into crystals for the determination of phases in the techniques of single (SIR) and multiple isomorphous replacement (MIR) which is needed for the determination of macromolecular crystal structures that lack a model suitable for molecular replacement (MR).

Crystallography soaking

How do you soak protein crystals?

What affects protein crystallization?

What is crystal soaking?

Does Aege soaking affect crystallographic structure?

What is Crystal soaking?

Why do macromolecular crystals need to be soaked?