Some of the ways a provider can collect cytology test samples include: Brushing or scraping tissue from the surface of a part of your body.
Collecting fluid or discharge samples from your body, such as a pee sample.
Using fine-needle aspiration to draw a fluid sample from an area in your body..
Papanicolau (pap) cytological staining is one of the most used procedures and comprises of several synthetic dyes that are either acidic (anionic) or basic (cationic) and with an affinity for the components of the cells.Mar 31, 2021.
Papanicolaou staining (Pap staining) is a widely used staining technique in cytopathology developed by Dr.
George N.
Papanicolaou.
We offer a series of pap staining dyes and solutions to differentially stain various components of the cells, allowing critical examination of nuclei and cytoplasmic components..
In the histopathology laboratory, the term “routine staining” refers to the hematoxylin and eosin stain (H&E) that is used “routinely” with all tissue specimens to reveal the underlying tissue structures and conditions..
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Many slides can be adjusted by returning a finished stained slide to the stain for deeper color reaction, or if overstained, can have some color removed by placing the slide in alcohol.
Formalin-fixed cytology preparations must be stained with either H&E or Papanicolaou stain.
The slides from the cell block are usually stained by a regular Hematoxylin and Eosin (HandE) stain.
These three types of stains are commonly used in different laboratories.
Each one of those has its advantages and disadvantages.
For example, DQ stain is good for microorganisms, cytoplasm, and background material staining.
This permits the formation of a stable bond between the slide and coverslip, and also protects the sample from air dry- ing or shrinkage and prevents oxidation or fading of the stain.
The universal stain for cytological samples is the PAP stain developed by the Greek cytopathologist, George Papanico- laou.
