The tissue obtained at the time of operation was crushed between the two clean glass slides with a pressure sufficient to spread the tissue into a film by the resident pathologist.
The smears were then fixed in 95% alcohol for 2 min and then stained with rapid hematoxylin and eosin staining method..
“Squash” preparations, where cells are intentionally squashed or crushed onto a slide to reveal their contents (e.g., botanical specimens where cells are disrupted to reveal chromosomes).
Conclusion Squash cytology is a rapid, inexpensive, and accurate diagnostic method for intraoperative diagnosis of gliomas that can guide the surgeon on the extent of tumor resection..
Squash smear cytology provides good cellular details and is far better for appreciating intercellular matrix tissue, inflammatory cells, lymphoma cells, or cells of pituitary adenoma, which are loosely arranged..
Squash smear preparation is a simple and rapid methodology for fairly accurate intraoperative diagnosis of CNS space occupying lesions [2-4].
The main goal of intraoperative cytodiagnosis in stereotactic biopsies is to confirm the adequacy of the tissue.Jul 1, 2016.
Squash smear preparation is a simple and rapid methodology for fairly accurate intraoperative diagnosis of CNS space occupying lesions [2-4].
The main goal of intraoperative cytodiagnosis in stereotactic biopsies is to confirm the adequacy of the tissue.Jul 1, 2016.
Squash smear preparation is a simple and rapid methodology for fairly accurate intraoperative diagnosis of CNS space occupying lesions [2-4].
The main goal of intraoperative cytodiagnosis in stereotactic biopsies is to confirm the adequacy of the tissue.Jul 1, 2016.
In view of the importance of squash cytology in diagnosis of intracranial lesions, the current study was planned to assess the feasibility and efficacy of intra-operative squash cytology as standalone method in the rapid intra-operative diagnosis of intracranial tumor and tumor-like lesions in setting where frozen section facility is not available.
For squash smear cytology [Figures 1 and 2 ], the diagnostic accuracy was 72% by complete concordance and increased to 90% on applying partial concordance criteria.
Neoplastic lesions showed better concordance than non-neoplastic lesions on applying complete concordance criteria.
Intraoperative squash smear cytology helps the surgeon to modify the extent of surgery.
Preoperative radiological investigation and intraoperative squash smear cytology are complementary to each other.
Both squash smear cytology and radiological investigations would have their own merits and demerits.
Space-occupying lesions (SOLs) of central nervous system (CNS) constitutes important cause of neurological morbidity and mortality. [ 1, 2] Squash cytology has been established as a technically simple, rapid, inexpensive, fairly accurate, and dependable intraoperative diagnostic tool for diagnosing CNS lesions. [ 3] .
