How do you stain a bone marrow smear?
To stain the bone marrow cells, immerse the slides in a Coplin jar containing May-Grunwald solution for 5 minutes.
Next, transfer the slides into a jar of buffer solution with a pH of 6.8 for 1 minute.
Then transfer the slides into Giemsa R solution for 10 minutes, followed by a 10-second wash with pH-neutral water..
What is BMA cytology?
Bone marrow aspiration (BMA) provides information about the numerical and cytological features of marrow cells, whereas bone marrow trephine biopsies (BMB) provide excellent appreciation of spatial relationships between cells and of overall bone marrow structure..
What is bone marrow iron stain?
The iron staining procedure utilizes the Prussian Blue stain for ferric iron to assess bone marrow iron stores.
This procedure is particularly helpful when evaluating patients with anemia, iron overload, myelodysplasia, etc..
What is the iron stain on a bone marrow biopsy?
Iron staining on bone marrow aspirate smears is commonly part of the standard order protocol for bone marrows aspirates.
The iron staining procedure utilizes the Prussian Blue stain for ferric iron to assess bone marrow iron stores..
What is the special stain for bone marrow biopsy?
Bone marrow sections should be routinely stained with haematoxylin and eosin (H&E) and a silver impregnation method for reticulin.
Sections can also be stained with Romanowsky dyes such as May–Gr\xfcnwald–Giemsa and for iron by Perls' reaction..
What stains are used for bone marrow examination?
Bone marrow films can be stained with a Romanowsky stain, such as May-Gr\xfcnwald-Giemsa, Wright-Giemsa, or aqueous-based Wright.
Because of their thickness, aspirate smears require a longer staining period with Romanowsky-type stains than blood films..
- During a bone marrow aspiration, your healthcare provider uses a needle to take a small sample of the liquid part of your bone marrow.
Your healthcare provider may order a bone marrow aspiration to evaluate your red blood cells (RBCs), white blood cells (WBCs) and platelets. - For the bone marrow aspiration, a thin needle is used to remove a sample of fluid (aspirate) from the bone marrow – usually from the pelvic bone, but sometimes from the chest.
A trephine biopsy, sometimes done with a second needle, removes a small piece of bone with the marrow inside. - Iron staining on bone marrow aspirate smears is commonly part of the standard order protocol for bone marrows aspirates.
The iron staining procedure utilizes the Prussian Blue stain for ferric iron to assess bone marrow iron stores.
