Wells prep cytology

How do you prepare a smear for cytology?
Smears are prepared from a small drop of the semisolid aspirate placed on a glass slide.
Advance the plunger of the syringe to express a small drop of the aspirated material on the center of the glass side..
Smears are prepared from a small drop of the semisolid aspirate placed on a glass slide.
Advance the plunger of the syringe to express a small drop of the aspirated material on the center of the glass side.
To prepare the cell block, the sample is aliquoted into the collodion lined tube and then centrifuged for 6-10 minutes at 1600-2500 rotations per minute (RCF 1125 g).
The supernatant is then carefully decanted or pipetted off (or can be removed later as the bag is being cut).

Apr 22, 2014How to take any sample containing cells, whether an aspirate from an organ or mass, or a fluid
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Can cytological preparations be used to diagnose solid tumors?

Molecular diagnostics has expanded to become the standard of care for a variety of solid tumor types.
With limited diagnostic material, it is often desirable to use cytological preparations to provide rapid and accurate molecular results.

How is effusion fluid prepared for cytopathologic evaluation?

Processing effusion fluid for cytopathologic evaluation.
Concentration of effusion fluid by centrifugation.
Pouring off supernatant and resuspending the cell pellet.
Preparation of direct smears.
In general, the direct smears prepared from concentrated fresh unfixed effusion specimens show flat cells with better cytomorphology.

What is the best approach for cell-blocking of cytology specimens?

For cell-blocking of cytology specimens, the best approach is to be compulsive and not to lose any cells or tissue micro-fragments in the specimen until the stage of getting tissue section on the glass slides.

Which cytology specimen should be provided on a glass slide?

Aspirate smears are the most commonly submitted cytology specimens and should be provided on fixed non-cover-slipped slides or from deparaffinizing 5–20-micron glass slide sections obtained from the corresponding FFPE cell blocks.

Wells prep cytology

How do you prepare a smear for cytology?

Can cytological preparations be used to diagnose solid tumors?

How is effusion fluid prepared for cytopathologic evaluation?

What is the best approach for cell-blocking of cytology specimens?

Which cytology specimen should be provided on a glass slide?