The main limitations of cytological samples are related to (1) the multitude of cytologic substrates and fixatives that require additional test validation as the majority of molecular assays are developed on FFPE tissue blocks, (2) the limited cellularity and the nucleic acid yield especially when the neoplastic cells Nov 22, 2018.
Material obtained analysed and interpreted Results and Conclusions: The procedure has two major limitations: non-diagnostic yield and indeterminate results..
The diagnostic accuracy of FNAC, however, may be lower in patients with primary lymphoproliferative disorders [7].
Early reports suggested that FNAC produced high false negative rates in patients with Hodgkin lymphoma (HL) and low-grade non-Hodgkin lymphoma..
Specifically, exfoliative cytology performed on oral cancers has high false negative rates, which can exceed 30%.
Furthermore, the effectiveness of exfoliative cytology for detecting dysplasia is even more doubtful, with false negative rates reported as high as 63%..
The main limitation of wide adoption of cytology specimens in molecular analysis is due to the need for additional test validation
The technological limitations of previous techniques, including the transmission of static digital images and dynamic streaming images, have limited telecytology to minor niches
In addition, a variety of cytology preparations provide less degraded material, faster fixation, as well as the option of immediate assessment, what ensures cellular adequacy and appropriate triaging of the sample, as opposed to histology samples
