Towards the design of an rna replicase

  • How does RNA replication take place?

    RNA-dependent RNA replication is a special process reserved exclusively for RNA viruses but not cellular RNAs.
    Almost all RNA viruses (except retroviruses) undergo RNA-dependent RNA replication by a virus-encoded RNA-dependent RNA polymerase (RdRP), which specifically replicates the viral RNA genome..

  • What are the steps of RNA replication?

    - RNA replication requires a DNA template for replication it does not replicate on its own. - RNA relocation just like DNA replication occurs in three stages that include initiation, elongation, and termination.
    Note: The RNAs are basically of three types depending upon the expression of the gene and their coding..

  • What is Q β replicase?

    Q beta replicase, on the other hand, amplifies a specific template molecule hybridized to target sequences and therefore amplifies a signal component of the system.
    For this reason, Q beta replicase amplification has applications in areas other than for the detection of nucleic acid sequences..

  • What is the replication of RNA called?

    RNA-dependent RNA replication is a special process reserved exclusively for RNA viruses but not cellular RNAs.
    Almost all RNA viruses (except retroviruses) undergo RNA-dependent RNA replication by a virus-encoded RNA-dependent RNA polymerase (RdRP), which specifically replicates the viral RNA genome..

  • Which direction is the template DNA read by the RNA polymerase?

    RNA polymerase synthesizes an RNA strand complementary to a template DNA strand.
    It synthesizes the RNA strand in the 5' to 3' direction, while reading the template DNA strand in the 3' to 5' direction..

  • Why RNA does not replicate?

    The 3′ sequences complementary to this region are found in the 3′ nontranslated region (see also Fig. 3.30).
    Changes in these sequences that eliminate cyclization prevent the RNA from replicating, even in model systems in which translation of the RNA is not required for expression of the replicase..

  • RNA fragments known as RNA primers play a critical role in initiating DNA replication by providing a binding site for the enzyme DNA polymerase, which creates the DNA copy.
  • The 3′ sequences complementary to this region are found in the 3′ nontranslated region (see also Fig. 3.30).
    Changes in these sequences that eliminate cyclization prevent the RNA from replicating, even in model systems in which translation of the RNA is not required for expression of the replicase.
First, the RNA must fold into the proper secondary structure so that the Pi stem forms; alternate secondary structures result in cleavage at incorrect sites (10) 
Towards the Design of an RNA Replicase. Abstract. The work in this thesis concentrates on the catalytic activities of two group I self-splicing RNA introns.

Do air-water interfaces enable RNA replication in a one-pot reaction?

Using a derivative of a group I intron as a model for an RNA replicase, we show that heated air-water interfaces that are exposed to a plausible CO 2 -rich atmosphere enable sense and antisense RNA replication as well as template-dependent synthesis and catalysis of a functional ribozyme in a one-pot reaction.

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Does co-replication of dominant RNA promote a multiple Replicator network?

Biochemical analyses supported the co-replication of dominant RNAs in the different lineages containing a cooperative RNA that replicates all other members, thus establishing a multiple replicator network.

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How does host RNA replicate in water-in-oil droplets?

The original host RNA replicates via translation of the self-encoded replicase, by which mutant host RNAs and parasitic RNAs could be generated. b Schematic representation of long-term replication experiments in water-in-oil droplets. (1) RNA replication was performed at 37 C for 5 h.

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What is self-replicating RNA derived from positive strand RNA viruses?

Contributor Information.
Self-replicating RNA derived from the genomes of positive strand RNA viruses represents a powerful tool for both molecular studies on virus biology and approaches to novel safe and effective vaccines.
The following chapter summarizes the principles how such RNAs can be established and used for design of vaccines.


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