Design and analysis qpcr

The guide starts with brief introduction to QPCR and experimental design

This is perhaps the most crucial step in the QPCR process as it lays the groundwork for every other aspect of the assay

A successful qPCR assay requires efficient and specific amplification of the product

Both the primers and the target sequence can affect amplification efficiency and specificity and thus the accuracy of qPCR assays

Therefore, care must be taken when choosing a target sequence and designing primers

The use of PCR primers specifically designed and validated for qPCR assays with your target of interest is highly recommended

This section provides information and tips on designing qPCR assay primers and probes, choosing a target sequence, multiplexing, selecting reporters and quenchers, and validating and optimizing qPCR assays