d) Disulfide bonds should be reduced with mercaptoethanol Page 2 B A peptide has the following amino acid composition: 2
all of the peptide bonds for which it is specific in one substrate The N-terminal amino acids present in digests were determined
The amino acids are joined by amide linkages called peptide bonds α carbon atom enzymes DNA polymerase replicates and repairs DNA transport proteins
amino acids bound by amide (peptide) bond C O NH peptides 2-100 amino acids natural peptides • product of proteolysis, enzymatic and nonenzymatic
amide bond (the carboxylic acids of one AA with the α-amino group of a second) enzymes involved in amino acid biosynthesis, metabolism and
The 20 standard amino acids found in protein structure are divided into seven This is due to increased exposure of peptide bonds to enzymes
peptide bond; this labilization (decrease in energy of activation) permits enzyme, D amino acids do inhibit 17 An explanation of this is apparent
enzyme bound 4'-phosphopantetheine (4'-PP) cofactor The nucleophilic attack of the hydroxy group of a highly conserved serine thioesterified amino acids