serial dilution lab report pdf


How to calculate the serial dilution?

    Calculate the final dilution ratio in a serial dilution. The total dilution ratio can be determined by multiplying the dilution factor of each step leading up to the final step. This can be mathematically illustrated with the equation D t = D 1 x D 2 x D 3 x … x D n where D t is the total dilution factor and D n is the dilution ratio.

Why is serial dilution necessary?

    serial dilutions are necessary to count cfus because they estimate the concentration (the number of bacteria or colonies) of a sample by diluting the original culture until you have a serial dilution that results in a countable number of colonies on a plate (usually between 30 to 300 cfus), which will be used to determine the cfu/ml count of the …

What does serial dilution mean?

    In a single and very simple word, Serial dilution is a laboratory technique, in which a stepwise dilution process is performed on a solution with an associated dilution factor. In the laboratory, this method is used to decrease the counts of cells within a culture to simplify the operation.
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