Biosynthesis of polyhydroxyalkanoates (PHAs) - ETH E - France


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PDF Biosynthesis of polyhydroxyalkanoates (PHAs) from low-cost growth

ETH No 22715 Biosynthesis of polyhydroxyalkanoates (PHAs) from low-cost growth carbon substrates in recombinant bacterial strains A thesis submitted to 

PDF Biosynthesis of Polyhydroxyalkanoates (PHAs) in Methane-utilizing

In this thesis the application of mixed culture and thermophilic biotechnology [3-6] are explored as means to potentially reduce production costs [3 7]

  • How are PHAs synthesized?

    PHAs are polyesters synthesized and stored by various bacteria and archaea in their cytoplasm as water-insoluble inclusions.
    PHAs are usually produced when the microbes are cultured with nutrient-limiting concentrations of nitrogen, phosphorus, sulfur, or oxygen and excess carbon sources.

  • What is the biosynthetic pathway of PHA?

    Metabolic pathways involved in Polyhydroxyalkanoate (PHA) synthesis from sugars, through glycolysis (a) or de novo fatty acid biosynthesis (b); from fatty acids, directly (c) or through fatty acid β-oxidation (d); and from alkanes, through alkane oxidation (e).

  • What is the production process of PHA?

    These authors describe a three-step PHA production process consisting of: .
    1) Feedstock production, where waste-based substrates are converted through anaerobic digestion to substrates for PHA production: organic and fatty acids. .
    2) Selection of PHA producing microbial cultures under changing feeding conditions.

  • 1 Mechanism of polyhydroxybutyrate synthesis.
    PHB biosynthesis by microbes occurs by the concentration of dual molecules of acetyl-CoA that would result in acetoacetyl-CoA which would be consequently condensed to hydroxybutyryl-CoA.
    This final complex would be utilized as a single unit for the synthesis of PHB.

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ETH No. 22715. Biosynthesis of polyhydroxyalkanoates (PHAs) from low-cost growth carbon substrates in recombinant bacterial strains. A thesis submitted to  Autres questions
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