Barcoding and molecular taxonomy as a multi-level tool


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PDF DNA barcoding: a way forward to obtain deep insights about

DNA barcoding is an innovative tool to rapidly identify and classify organisms based on highly conserved species-specific DNA sequences This technique has developed in parallel with other genomic-based investigations which share special emphasis on the acquisition of data pertaining to hotspots regions of DNA (usually short sequence of DNA up to

PDF DNA barcoding: how it complements taxonomy molecular

barcoding datasets are essentially composed of short DNA sequences from several individuals of a large number of species (typically five to ten individuals per species but these numbers will increase in the future) (Figure 1)

  • Does DNA barcoding reveal cryptic species in the Neotropical skip-Per butterfly?

    Hebert PD, Penton EH, Burns JM, et al. Ten species in one: DNA barcoding reveals cryptic species in the neotropical skip-per butterfly Astraptes fulgerator. Proc Natl Acad Sci USA. 2004;101(41):14812–7. 39. Hebert PDN, Cywinska A, Ball SL, et al. Biological identifica-tions through DNA barcodes. Proc R Soc Lond Ser B Biol Sci. 2003;270:313–21.

  • Is DNA barcoding effective in molecular taxonomy?

    Purty RS, Chatterjee S (2016) DNA barcoding: an effective technique in molecular taxonomy. Austin J Biotechnol Bioeng 3:1059 Schneider KL, Marrero G, Alvarez AM, Presting GG (2011) Classification of plant associated bacteria using RIF, a computationally derived DNA marker.

  • What are the global DNA barcoding efforts in the marine habitat?

    Global DNA barcoding efforts in the marine habitat include MarBOL, CeDAMar, CMarZ, SHARK-BOL, etc. An overview on DNA barcoding of different marine groups ranging from the microbes to mammals is revealed.

  • Which DNA barcodes are used for species identification?

    Several DNA barcodes have been extensively used for biological species identification, including the mitochondrial COI gene, rbcL, matK, trnH-psbA, 16S rRNA, V4, D1–D2 region, and ITS (nuclear internal transcribed spacer). But there is no single barcode for all the species and it is very hard to find because of differences in evolutionary rates.

Abstract

DNA barcoding is an innovative tool to rapidly identify and classify organisms based on highly conserved species-specific DNA sequences. This technique has developed in parallel with other genomic-based investigations, which share special emphasis on the acquisition of data pertaining to hotspots regions of DNA (usually short sequence of DNA up to

Future prospectus of DNA barcoding

DNA barcoding has been widely used to barcode living organisms. Currently more than million barcodes have been generated in the BOLD system (DNA barcode data deposi-tory). The barcoding is further accelerated by iBOL to create huge libraries for facilitating proper identification of living organisms. In addition to its role in taxonomic studies, ba

Compliance with ethical standards

Conflict of interest On behalf of all authors, the corresponding author states that there is no conflict of interest. link.springer.com

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