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Keller-Kiliani test 3 1 2 Kedde-test 3 1 3 Baljet-test 3 1 4 Legal-test Use 5 ml of the extract for test-tube reaction (3 1 ) and investigate its 10 

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Identification of cardiac glycosides Page 15 15 • Tests for sugar part – Keller-Kiliani test: with glacial acetic acid with traces of ferric (III) chloride 

  • Keller kiliani test: this test is specific for digitoxose moiety. 2.
    Baljet test: This comprises sodium picrate solution.
    A yellow color is observed which changes to orange.

  • What is the identification test for deoxy sugar?

    Keller-Kiliani Test for Deoxysugars
    A Deoxysugar (found in cardiac glycosides) is dissolved in acetic acid containing a trace of ferric chloride and trans-ferred to the surface of concentrated sulphuric acid.
    At the junction of the liquids a reddish-brown colour is produced which gradually becomes blue.

  • What is the Keller Kiliani test for digitoxin?

    The test is based on the specificity of action of the acid hydroliysis of deoxy-sugars like digitoxin (glycoside) that is transformed to digitoxigenin (aglycone) and 3-digitoxose (sugar residue) and eventually cymarose. digitoxin is obtained at first by alkaline hydrolysis from acetyldigitoxin.

  • What is the reagent in the Keller Kiliani test?

    Keller-Kiliani Test.
    A solution of glacial acetic acid (4.0 ml) with 1 drop of 2.0% FeCl3 mixture was mixed with the 10 ml aqueous plant extract and 1 ml H2SO4 concentrated.
    A brown ring formed between the layers which showed the entity of cardiac steroidal glycosides.

  • Explanation: Keller's reagent is the find different kinds of Alkaloids via reactions, which produced the products with the colors. The Cohn defined the theory, its use to identify, the principal components of digitalis.so the reaction is known as Keller–Kiliani reaction.
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