methods of fixation


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Biological electron microscopy generally employs two methods for obtaining these results chemical and/or physical fixation While chemical fixation remains 

  • How many types of fixative are there?

    Common methods of fixation include: Perfusion: Tissues can be perfused with fixative following exsanguination and saline perfusion to allow rapid fixation of entire organs.
    Immersion: Samples are immersed in fixative which then diffuses into and through the tissue or cell sample.

  • What are the 3 types of fixation?

    5.
    5) TYPE OF FIXATION
    The most commonly used technique is simple immersion of tissues/smears in an excess of fixative.
    For all practical purposes immersion fixatives are most useful.
    These may be divided into routine and special.

  • What is the most commonly used fixation technique?

    There are three broad types of compound fixatives.
    Microanatomical, Cytological and Histochemical fixatives.

Types of fixation Fixation of tissues can be achieved by chemical or physical means. Physical methods include heating, micro-waving and cryo-preservation (freeze drying). Heat fixation is rarely used on tissue specimens, its application being confined to smears of micro organisms.
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