rocking microtome

What is a rocking microtome?
The rocking microtome is a device for cutting thin sections of wax-embedded material of either animal or plant origin. The rocking microtome was developed in workshops of Cambridge University at the end of XIX century. Though it is an old model, it is still very effective for producing series of good quality 6-20 micron thin paraffin sections.
How does a microtome work?
The microtome consists of the rocking arm (1) and the micrometer screw (2) operated by the lever (3) installed on a cast-iron base. The sample is mounted on a holder and the holder is attached to its base (5) at the end of the rocking arm (1) and tightened using the screw (7). Then the knife is fit into the holder (4) and locked by the screw (6).
Why does a microtome not provide a flat section?
1. Tissue is curved and the microtome does not provide flat section. 2. As the microtome is of light weight so vibration may occur. Base sledge microtome: In sledge microtome the block is fixed in a static position within a steel carriage. The knife slides to and fro over the top of the block.
What kind of knife does a rocking microtome use?
The rocking microtome uses the A-type biconcave razor knives. These knives are quite easy to sharpen but also quite rapidly get blunt. I believe this is the only type of the knife that can be used in this microtome model.

Microtome Knife

The microtome knife is important to cut uniform and thin section of tissue (Box 5.1). These are made of steel. Various types of knife profiles are available for different types of microtomes. The most commonly used knife profile is Profile C or wedge profile. The various types of microtome knife include (Fig. 5.3): 1. 1. Plano concave (Profile A):

Disposable Knife

Nowadays disposable blade is used in many laboratories to save time to sharpen. Two types of disposable blades are available: 1. 1. Low-profile blade: These blades are used to cut small biopsy or soft large tissue. 2. 2. High-profile blade: These are used to cut firm to relatively hard tissue such as the uterus, heart, etc. Advantages: 2.1. 1. Easy

Materials Used in Knife

Conventional knife: The conventional microtome knifes are made of very high-quality carbon or steel material that are usually tempered from the edge to inside one third of the width. The desirable hardness of the knife is in between 400 and 900 Vickers hardness scale. The diamond knife: This is a costly knife and is used to cut epoxy resin blocks i

Angles of Knife

The various angles formed by the knife include (Fig. 5.4): Rake angle: It is the angle between the upper bevel of the knife and the perpendicular line drawn from the surface of the block. Increased rake angle makes the section cutting easier. Angle of clearance: It is the angle between the lower bevel of the knife and the surface of the block. It i

5.1 Microtome Knives

Types based on shape: 1. Plano concave (Profile A): 1.1. Very sharp knife and is used for cutting soft tissues 2. Biconcave (Profile B): 2.1. Used for rocking microtome 2.2. Vibrates during cutting 3. Wedge (Profile C): 3.1. Most commonly used 4. Tool edge (Profile D): 4.1. Used for hard tissue Types based on disposability: 1. Permanent 2. Disposab

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