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PDF Assessment of total phenolic and flavonoid contents antioxidant

Assessment of total phenolic and flavonoid contents antioxidant activity and Test for flavonoids (Shinoda's test) The test solution of the extracts was 

  • Lead-acetate test : Add few drops of aqueous basic lead acetate solution to 1 mL of alcoholic extract using a test tube.
    The appearance of a reddish brown bulky precipitate indicates the presence of flavonoids.

  • What is the principle of ammonia test for flavonoids?

    First, dilute ammonia (5 ml) was added to a portion of an aqueous filtrate of the extract.
    Concentrated sulphuric acid (1 ml) was added.
    A yellow colouration that disappear on standing indicates the presence of flavonoids.

  • What reagent is used for flavonoids?

    For flavonoid compounds, 5% ethanolic aluminum chloride, 5% aqueous sodium carbonate, lo/, aqueous ferric chloride + 1% . aqueous potassium ferricyanide (1 : 1). 1o/0 aqueous sodium hydroxide, p-toluenesulfonic acid and ammonia vapor are commonly used3*“.

  • How do you test for flavonoids in Shinoda?

    Test for flavonoids (Shinoda's test)
    The test solution of the extracts was dissolved in 95% ethanol.
    To this, a small piece of magnesium foil metal was added; this was followed by 3-5 drops of the concentrated HCl.
    The intense cherry red color indicated the presence of flavonoids.

  • Tests for flavonoids (a) Alkaline reagent test. Two to three drops of sodium hydroxide were added to 2 mL of extract. Initially, a deep yellow colour appeared but it gradually became colourless by adding few drops of dilute HCL, indicating that flavonoids were present. (b) Shinod's test.
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