acid hydrolysis fat determination


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PDF ILIADe 179:2023 CLEN Method

14 oct 2019 · This method can be applied for the analysis of food where oils and fats cannot be extracted without a previous hydrolysis except for the case 

  • Lipid hydrolysis produces glycerol and fatty acids.

  • What is the fat hydrolysis method?

    Hydrolysis of oils and fats is the applied term to the operation in which ethanolic KOH reacts with oil to form glycerol and fatty acids (FAs).
    Production of FAs and glycerol from oils are important especially in oleochemical industries.

  • What is the acid hydrolysis method of fat analysis?

    Answer: This method is used to measure crude fat by acid hydrolysis.
    The fat is released with hydrochloric acid and is extracted with ethyl ether and hexane.
    It is then filtered, dried, and gravimetrically measured.
    This method is used for a variety of foods.

  • Crude fat is determined by acid hydrolysis of the sample with HCl (25 + 11) followed by extraction of hydrolyzed lipid materials with mixed ethers. Ethers are evaporated, and lipid residue is heated to constant weight at 100°.
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